Skip to main content
SpringerLink
Log in

PCR analysis of HBV infected sera: relationship between expression of pre-S antigens and viral replication

  • Conference paper
Chronically Evolving Viral Hepatitis

Part of the book series: Archives of Virology ((ARCHIVES SUPPL,volume 4))

Summary

In order to determine the biological significance of the pre-S antigens in HBV infection, HBsAg sera were tested for the presence of pre-S 1 and pre-S2. HBV DNA was detected by spot-hybridization and PCR. The data show a complete correlation between pre-S antigenemia and HBV DNA replication in anti-HBe positive cases. PCR but not spot-hybridization was adequately sensitive to also detect HBV DNA in roughly half of the preS negative sera as well. Thus PCR appears to be a valuable technique for detection of potentially infectious anti-HBe carriers.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Neurath AR, Kent SBH (1988) Adv Virus Res 34: 65–142

    Article  PubMed  CAS  Google Scholar 

  2. Budkowska A et al (1986) Hepatology 6: 360–368

    Article  PubMed  CAS  Google Scholar 

  3. Carloni G et al (1987) J Med Virol 21: 15–23

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Cell Biology, Istituto Superiore di Sanità, I-00161, Rome, Italy

    Dr. A. R. Garbuglia & C. Delfini

  2. Institute of Microbiology, University of Ancona, Italy

    A. Manzin

  3. Institut Pasteur, Paris, France

    A. Budkowska

  4. Institute of Infectious Diseases, University of Rome, Italy

    G. Taliani

  5. Institute of Experimental Medicine, C.N.R., Rome, Italy

    G. Carloni

Authors
  1. Dr. A. R. Garbuglia
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Manzin
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. Budkowska
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. G. Taliani
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. C. Delfini
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. G. Carloni
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Titolare II Cattedra Clinica, Malattie Tropicli e Infettive, Policlinico Umberto, Roma, Italy

    C. De Bac  & G. Taliani  & 

  2. Institut für Medizinische Virologie, Justus-Leibig-Universität, Giessen, Federal Republic of Germany

    W. H. Gerlich

Rights and permissions

Reprints and permissions

Copyright information

© 1992 Springer-Verlag

About this paper

Cite this paper

Garbuglia, A.R., Manzin, A., Budkowska, A., Taliani, G., Delfini, C., Carloni, G. (1992). PCR analysis of HBV infected sera: relationship between expression of pre-S antigens and viral replication. In: De Bac, C., Taliani, G., Gerlich, W.H. (eds) Chronically Evolving Viral Hepatitis. Archives of Virology, vol 4. Springer, Vienna. https://doi.org/10.1007/978-3-7091-5633-9_23

Download citation

  • DOI: https://doi.org/10.1007/978-3-7091-5633-9_23

  • Publisher Name: Springer, Vienna

  • Print ISBN: 978-3-211-82350-7

  • Online ISBN: 978-3-7091-5633-9

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation