Abstract
Photosynthesis is the process to convert solar energy to biomass and biofuels, which are the only major solar energy storage means on Earth. To satisfy the increased demand for sustainable energy sources, it is essential to understand the process of solar energy storage, that is, the carbon metabolism in photosynthetic organisms. It has been well-recognized that one bottleneck of photosynthesis is carbon assimilation. In this report, we summarize our recent studies on the carbon metabolism pathways of several types of photosynthetic bacteria, including aerobic anoxygenic phototrophic proteobacteria, green sulfur bacteria, heliobacteria and cyanobacteria, using physiological studies, transcriptomics, enzyme assays, 13C-based metabolomics and fluxomics. Our studies have revealed several unique and/or significant central carbon metabolic pathways and novel enzymes that operate in these phototrophs, quantified CO2 assimilation pathways operative during mixotrophic cultivation conditions, and also suggested evolutionary links between photosynthetic and non-photosynthetic organisms.
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References
Atsumi S, Liao JC (2008) Directed Evolution of Methanococcus Jannaschii Citramalate Synthase for Biosynthesis of 1-Propanol and 1-Butanol by Escherichia Coli. Appl Environ Microbiol 74: 7802–7808
Blankenship RE (2002) Molecular Mechanisms of Photosynthesis. Blackwell Science Ltd, Oxford
Conrad R, Schlegel HG (1977) Different Degradation Pathways for Glucose and Fructose in Rhodopseudomonas Capsulata. Arch Microbiol 112: 39–48
Conway T (1992) The Entner-Doudoroff Pathway: History, Physiology and Molecular Biology. FEMS Microbiol Rev 9: 1–27
Feng X, Bandyopadhyay A, Berla B, Page L, Wu B, Pakrasi HB, Tang YJ (2010a) Mixotrophic and Photoheterotrophic Metabolism in Cyanothece sp. ATCC 51142 under Continuous Light. Microbiology 156: 2566–2574
Feng X, Mouttaki H, Lin L, Huang R, Wu B, Hemme CL, He Z, Zhang B, Hicks LM, Xu J, Zhou J, Tang YJ (2009) Characterization of the Central Metabolic Pathways in Thermoanaerobacter sp. X514 Via Isotopomer-Assisted Metabolite Analysis. Appl. Environ. Microbiol. 75: 5001–5008
Feng X, Tang KH, Blankenship RE, Tang YJ (2010b) Metabolic Flux Analysis of the Mixotrophic Metabolisms in the Green Sulfur Bacterium Chlorobaculum Tepidum. J Biol Chem 285: 39544–39550
Fuhrer T, Fischer E, Sauer U (2005) Experimental Identification and Quantification of Glucose Metabolism in Seven Bacterial Species. J Bacteriol 187: 1581–1590
Furch T, Preusse M, Tomasch J, Zech H, Wagner-Dobler I, Rabus R, Wittmann C (2009) Metabolic Fluxes in the Central Carbon Metabolism of Dinoroseobacter Shibae and Phaeobacter Gallaeciensis, Two Members of the Marine Roseobacter clade. BMC Microbiol 9: 209
Howell DM, Xu H, White RH (1999) (R)-Citramalate Synthase in Methanogenic Archaea. J Bacteriol 181: 331–333
Kramer DM, Schoepp B, Liebl U, Nitschke W (1997) Cyclic Electron Transfer in Heliobacillus Mobilis Involving a Menaquinol-Oxidizing Cytochrome bc Complex and an RCI-Type Reaction Center. Biochemistry 36: 4203–4211
Li F, Hagemeier CH, Seedorf H, Gottschalk G, Thauer RK (2007) Re-Citrate Synthase from Clostridium Kluyveri Is Phylogenetically Related to Homocitrate Synthase and Isopropylmalate Synthase Rather than to Si-Citrate Synthase. J Bacteriol 189: 4299–4304
Molenaar D, vanBerlo R, de Ridder D, Teusink B (2009) Shifts in Growth Strategies Reflect Tradeoffs in Cellular Economics. Mol Syst Biol 5: 323
Pickett MW, Williamson MP, Kelly DJ (1994) An Enzyme and 13C-NMR of Carbon Metabolism in Heliobacteria. Photosynth. Res. 41: 75–88
Pingitore F, Tang Y, Kruppa GH, Keasling JD (2007) Analysis of Amino Acid Isotopomers Using FT-ICR MS. Anal Chem 79: 2483–2490
Risso C, Van Dien SJ, Orloff A, Lovley DR, Coppi MV (2008) Elucidation of an Alternate Isoleucine Biosynthesis Pathway in Geobacter Sulfurreducens. J Bacteriol 190: 2266–2274
Sattley WM, Blankenship RE (2009) Insights into Heliobacterial Photosynthesis and Physiology from the Genome of Heliobacterium Modesticaldum. Photosynth. Res
Sattley WM, Madigan MT, Swingley WD, Cheung C, Clocksin KM, Conrad AL, Dejesa LC, Honchak BM, Jung DO, Karbach LE, Kurdoglu A, Lahiri S, Mastrian SD, Page LE, Taylor HL, Wang ZT, Raymond J, Chen M, Blankenship RE, Touchman JW (2008) The Genome of Heliobacterium Modesticaldum, a Phototrophic Representative of the Firmicutes Containing the Simplest Photosynthetic Apparatus. J Bacteriol 190: 4687–4696
Tang KH, Blankenship RE (2010) Both Forward and Reverse TCA Cycles Operate in Green Sulfur Bacteria. J Biol Chem 285: 35848–35854
Tang KH, Feng X, Tang YJ, Blankenship RE (2009a) Carbohydrate Metabolism and Carbon Fixation in Roseobacter Denitrificans OCh1 14. PLoS One 4: e7233
Tang KH, Feng X, Zhuang WQ, Alvarez-Cohen L, Blankenship RE, Tang YJ (2010a) Carbon Flow of Heliobacteria Is Related More to Clostridia than to the Green Sulfur Bacteria. J Biol Chem 285: 35104–35112
Tang KH, Wen J, Li X, Blankenship RE (2009b) Role of the AcsF Protein in Chloroflexus Aurantiacus. J Bacteriol 191: 3580–3587
Tang KH, Yue H, Blankenship RE (2010b) Energy Metabolism of Heliobacterium Modesticaldum during Phototrophic and Chemotrophic Growth. BMC Microbiol 10: 150
Thauer RK (2007) Microbiology. A Fifth Pathway of Carbon Fixation. Science 318: 1732–1733
Wahl SA, Dauner M, Wiechert W (2004) New Tools for Mass Isotopomer Data Evaluation in (13)C Flux Analysis: Mass Isotope Correction, Data Consistency Checking, and Precursor Relationships. Biotechnol Bioeng 85: 259–268
Wu B, Zhang B, Feng X, Rubens JR, Huang R, Hicks LM, Pakrasi HB, Tang YJ (2010) Alternative Isoleucine Synthesis Pathway in Cyanobacterial Species. Microbiology 156: 596–602
Zhu XG, Long SP, Ort DR (2010) Improving Photosynthetic Efficiency for Greater Yield. Annu Rev Plant Biol 61: 235–261
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© 2013 Zhejiang University Press, Hangzhou and Springer-Verlag Berlin Heidelberg
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Tang, KH., Feng, X., Bandyopadhyay, A., Pakrasi, H.B., Tang, Y.J., Blankenship, R.E. (2013). Unique Central Carbon Metabolic Pathways and Novel Enzymes in Phototrophic Bacteria Revealed by Integrative Genomics, 13C-based Metabolomics and Fluxomics. In: Photosynthesis Research for Food, Fuel and the Future. Advanced Topics in Science and Technology in China. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-32034-7_71
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DOI: https://doi.org/10.1007/978-3-642-32034-7_71
Publisher Name: Springer, Berlin, Heidelberg
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