Abstract
Phosphoglycolate phosphatase (PGPase) is an essential enzyme in the photorespiration pathway in plants and photosynthetic algae. In Chlamydomonas reinhardtii, mutants deficient in phosphoglycolate phosphatase (PGPase-1) were reported to require elevated levels of CO2 for growth in the light. Here we described that thispgp1 mutant reverted naturally and regained the ability to survive under low CO2 conditions. Quantitative RT-PCR analysis demonstrated that the upregulation of PGP2 in the pgp1 revertants might contribute to this reversion in the growth phenotype.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Anderson LE (1971) Chloroplast and Cytoplasmic Enzymes. Ii. Pea Leaf Triose Phosphate Isomerases. Biochim Biophys Acta 235: 237–244
Husic HD, Tolbert NE (1985) Properties of Phosphoglycolate Phosphatase from Chlamydomonas Reinhardtii and Anacystis Nidulans. Plant Physiol 79: 394–399
Moroney JV, Wilson BJ, Tolbert NE (1986) Glycolate Metabolism and Excretion by Chlamydomonas Reinhardtii. Plant Physiol 82: 821–826
Rawat M, Moroney JV (1991) Partial Characterization of a New Isoenzyme of Carbonic Anhydrase Isolated from Chlamydomonas Reinhardtii. J Biol Chem 266: 9719–9723
Suzuki K (1995) Phosphoglycolate Phosphatase-Defident Mutants of Chlamydomonas Reinhardtii Capable of Growth under Air. Plant And Cell Physiology 36: 95–100
Suzuki K, Marek LF, Spalding MH (1990) A Photorespiratory Mutant of Chlamydomonas Reinhardtii. Plant Physiol 93: 231–237
Suzuki K, Uchida H, Mamedov TG (2005) The Phosphoglycolate Phosphatase Gene and the Mutation in the Phosphoglycolate Phosphatase-Deficient Mutant (Pgp1-1) of Chlamydomonas Reinhardtii. Canadian Journal Of Botany 83: 842–849
Tolbert NE (1997) The C2 Oxidative Photosynthetic Carbon Cycle. Annu Rev Plant Physiol Plant Mol Biol 48: 1–25. Doi:10.1146/Annurev.Arplant.48.1.1
Ynalvez RA, Xiao Y, Ward AS, Cunnusamy K, Moroney JV (2008) Identification and Characterization of Two Closely Related B-Carbonic Anhydrases from Chlamydomonas Reinhardtii. Physiologia Plantarum 133: 15–26
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
Copyright information
© 2013 Zhejiang University Press, Hangzhou and Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Ma, Y., Hartman, M.M., Moroney, J.V. (2013). Transcriptional Analysis of the Three Phosphoglycolate Phosphatase Genes in Wild Type and the pgp1 Mutant of Chlamydomonas Reinhardtii . In: Photosynthesis Research for Food, Fuel and the Future. Advanced Topics in Science and Technology in China. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-32034-7_66
Download citation
DOI: https://doi.org/10.1007/978-3-642-32034-7_66
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-32033-0
Online ISBN: 978-3-642-32034-7
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)