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Agrobacterium-Mediated Transformation: Rice Transformation

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Cereal Genomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1099))

Abstract

Agrobacterium is a common soil bacterium with natural capacity for trans-kingdom transfer of genetic information by transferring its T-DNA into the eukaryotic genome. In agricultural plant biotechnology, combination of non-phytopathogenic strain of Agrobacterium tumefaciens with modified T-DNA and vir-genes in a binary vector system is the most widely utilized system for genetic improvement in diverse plant species and for gene function validation. Here we have described a highly efficient A. tumefaciens-mediated transformation system for indica and japonica rice cultivars based on an immature embryo system.

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Acknowledgement

Establishment of the protocol was supported by USAID grant number DPPC2004-17. Technical support of F. Montecillo and facilitation of P. Herve and G. Barry are acknowledged.

Author information

Authors and Affiliations

  1. Genetic Transformation Laboratory, Plant Breeding, Genetics, and Biotechnology, International Rice Research Institute, Metro Manila, Philippines

    Inez H. Slamet-Loedin

  2. Plant Breeding, Genetics, and Biotechnology, International Rice Research Institute, Metro Manila, Philippines

    Prabhjit Chadha-Mohanty & Lina Torrizo

Authors
  1. Inez H. Slamet-Loedin
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  2. Prabhjit Chadha-Mohanty
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  3. Lina Torrizo
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Editor information

Editors and Affiliations

  1. Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St. Lucia, Queensland, Australia

    Robert J. Henry

  2. Centre for Nutrition and Food Science, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Lismore, New South Wales, Australia

    Agnelo Furtado

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© 2014 Springer Science+Business Media New York

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Slamet-Loedin, I.H., Chadha-Mohanty, P., Torrizo, L. (2014). Agrobacterium-Mediated Transformation: Rice Transformation. In: Henry, R., Furtado, A. (eds) Cereal Genomics. Methods in Molecular Biology, vol 1099. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-715-0_21

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  • DOI: https://doi.org/10.1007/978-1-62703-715-0_21

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-714-3

  • Online ISBN: 978-1-62703-715-0

  • eBook Packages: Springer Protocols

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