Abstract
Mouse models of systemic anaphylaxis are important tools for the study of mast cell function, for the elucidation of the pathomechanisms of anaphylaxis, and for identifying and characterizing potential therapies for anaphylaxis. Here, we describe two murine models of systemic anaphylaxis that have been a key part of research in these areas. In a passive model, mice are sensitized with antigen-specific IgE antibody 24 h prior to antigen challenge. In an active model, mice are instead sensitized with antigen 18–21 days prior to challenge. Hypothermia serves as the primary quantifiable indicator of anaphylaxis in these models.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Summers CW, Pumphrey RS, Woods CN et al (2008) Factors predicting anaphylaxis to peanuts and tree nuts in patients referred to a specialist center. J Allergy Clin Immunol 121:632–638.e632
Lee JK, Vadas P (2011) Anaphylaxis: mechanisms and management. Clin Exp Allergy 41:923–938
Sicherer SH, Leung DY (2012) Advances in allergic skin disease, anaphylaxis, and hypersensitivity reactions to foods, drugs, and insects in 2011. J Allergy Clin Immunol 129:76–85
Metz M, Schäfer B, Tsai M et al (2011) Evidence that the endothelin A receptor can enhance IgE-dependent anaphylaxis in mice. J Allergy Clin Immunol 128:424–426.e1
Freichel M, Almering J, Tsvilovskyy V (2012) The role of TRP proteins in mast cells. Front Immunol 3:150
Khan BQ, Kemp SF (2011) Pathophysiology of anaphylaxis. Curr Opin Allergy Clin Immunol 11:319–325
Vöhringer D (2011) Basophils in allergic immune responses. Curr Opin Immunol 23:789–793
Finkelman FD (2007) Anaphylaxis: lessons from mouse models. J Allergy Clin Immunol 120:506–515
Ando A, Martin TR, Galli SJ (1993) Effects of chronic treatment with the c-kit ligand, stem cell factor, on immunoglobulin E-dependent anaphylaxis in mice. Genetically mast cell-deficient Sl/Sld mice acquire anaphylactic responsiveness, but the congenic normal mice do not exhibit augmented responses. J Clin Invest 92:1639–1649
Metz M, Piliponsky AM, Chen CC et al (2006) Mast cells can enhance resistance to snake and honeybee venoms. Science 313:526–530
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Doyle, E., Trosien, J., Metz, M. (2013). Protocols for the Induction and Evaluation of Systemic Anaphylaxis in Mice. In: Allen, I. (eds) Mouse Models of Allergic Disease. Methods in Molecular Biology, vol 1032. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-496-8_10
Download citation
DOI: https://doi.org/10.1007/978-1-62703-496-8_10
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-495-1
Online ISBN: 978-1-62703-496-8
eBook Packages: Springer Protocols