Abstract
The study of transcriptome responses can provide insight into the regulatory pathways and genetic factors that contribute to a specific phenotype. For bacterial pathogens, it can identify putative new virulence systems and shed light on the mechanisms underlying the regulation of virulence factors. Microarrays have been previously used to study gene regulation in Legionella pneumophila. In the past few years a sharp reduction of the costs associated with microarray experiments together with the availability of relatively inexpensive custom-designed commercial microarrays has made microarray technology an accessible tool for the majority of researchers. Here we describe the methodologies to conduct microarray experiments from in vitro and in vivo samples.
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References
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Acknowledgments
Work in the authors’ laboratory was supported by a start-up fund from McGill University and National Sciences and Engineering Research Council of Canada (NSERC) Discovery grant RGPIN 418289-12 (S.P.F) and PHS award AI064481 (H.A.S).
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Faucher, S.P., Shuman, H.A. (2013). Methods to Study Legionella Transcriptome In Vitro and In Vivo. In: Buchrieser, C., Hilbi, H. (eds) Legionella. Methods in Molecular Biology, vol 954. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-161-5_35
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DOI: https://doi.org/10.1007/978-1-62703-161-5_35
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-160-8
Online ISBN: 978-1-62703-161-5
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