Abstract
Lipid analysis has been a crucial source of information in cystic fibrosis (CF). New methodologies for qualitative and quantitative lipidomics allow evaluation of a large number of samples, of special interest in patient screening for diagnostic and prognostic biological markers, as well as in cell physiology. In this chapter, two new complementary approaches are described: matrix-assisted laser desorption coupled to time of flight (MALDI-TOF-ClinProTools™) and liquid chromatography coupled to ion trap mass spectrometry (LC-MSn). MALDI-TOF-ClinProTools™ offers a large unbiased screening for the discovery of potential lipid alterations in diseased patients. LC-MSn represents a state-of-the-art lipidomic tool for the identification and quantification of such alterations. The combination of both may open new perspectives in the quest for lipids participating in CF pathogenesis, therapy targets, and biomarkers.
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Acknowledgments
This work was supported by NEUPROCF (FP6, European Commission to A.E.) and EICOCF (Association Nationale de la Recherche to A.E.), the associations Vaincre la Mucoviscidose and ABCF2 Protéines, and Legs Poix-University of Paris 5. The authors thank the Agilent Technologies/University of California Irvine Analytical Discovery Facility, the Center for Drug Discovery, and the Agilent Technologies Foundation.
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Ollero, M., Guerrera, I.C., Astarita, G., Piomelli, D., Edelman, A. (2011). New Lipidomic Approaches in Cystic Fibrosis. In: Amaral, M., Kunzelmann, K. (eds) Cystic Fibrosis. Methods in Molecular Biology, vol 742. Humana Press. https://doi.org/10.1007/978-1-61779-120-8_16
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DOI: https://doi.org/10.1007/978-1-61779-120-8_16
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