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Cystic Fibrosis pp 255–270Cite as

Analysis of CFTR Interactome in the Macromolecular Complexes

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 741))

Abstract

Cystic fibrosis transmembrane conductance regulator (CFTR) is a chloride channel localized primarily at the apical surface of epithelial cells lining the airway, gut, exocrine glands, etc., where it is responsible for transepithelial salt and water transport. A growing number of proteins have been reported to interact directly or indirectly with CFTR chloride channel, suggesting that CFTR might regulate the activities of other ion channels, receptors, and transporters, in addition to its role as a chloride conductor. Most interactions occur primarily between the opposing terminal tails (N or C) of CFTR and its binding partners, either directly or mediated through various PDZ domain-containing proteins. This chapter describes methods we developed to cross-link CFTR into a macromolecular complex to identify and analyze the assembly and regulation of CFTR-containing complexes in the plasma membrane.

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Acknowledgments

We thank Dr David Armbruster for critically reading the manuscript and Ms. Feng Zhou for formatting the references. This work was supported by National Institute of Diabetes and Digestive and Kidney Diseases grants DK058545 and DK074996 (to A.P.N.), and American Heart Association (Midwest Affiliate) Beginning-grant-in-aid #0765185B (to C.L.).

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Wayne State University School of Medicine, 48201, Detroit, MI, USA

    Chunying Li

  2. Department of Physiology, University of Tennessee Health Science Center, 38163, Memphis, TN, USA

    Anjaparavanda P. Naren

Authors
  1. Chunying Li
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  2. Anjaparavanda P. Naren
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Correspondence to Chunying Li .

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Editors and Affiliations

  1. Faculty of Sciences, Centre for Biodiversity & Functional, University of Lisboa, Campo Grande, Lisboa, 1749-016, Portugal

    Margarida D. Amaral

  2. , Department of Physiology, University of Regensburg, Universitätsstr. 31, Regensburg, 93053, Germany

    Karl Kunzelmann

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Li, C., Naren, A.P. (2011). Analysis of CFTR Interactome in the Macromolecular Complexes. In: Amaral, M., Kunzelmann, K. (eds) Cystic Fibrosis. Methods in Molecular Biology, vol 741. Humana Press. https://doi.org/10.1007/978-1-61779-117-8_17

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  • DOI: https://doi.org/10.1007/978-1-61779-117-8_17

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-116-1

  • Online ISBN: 978-1-61779-117-8

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