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Yeast Genetic Networks pp 317–333Cite as

Quantitation of Cellular Components in Cryptococcus neoformans for System Biology Analysis

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 734))

Abstract

Methods and procedures in molecular biology used to study fungal pathogenesis have significantly improved during the last decade. In this chapter, we provide step-by-step procedures for performing genetics and biochemical studies in the human pathogenic fungal microorganism Cryptococcus neoformans (Cn). These methods are employed for studying the pathobiology of Cn and for experimental validation of theoretical models of fungal pathogenicity.

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Acknowledgments

This work was supported by Grants AI56168 and AI72142 (to M.D.P) and was conducted in a facility constructed with support from the National Institutes of Health, Grant Number C06 RR015455 from the Extramural Research Facilities Program of the National Center for Research Resources. Dr. Maurizio Del Poeta is a Burroughs Wellcome New Investigator in Pathogenesis of Infectious Diseases.

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Authors and Affiliations

  1. Department of Biochemistry, Medical University of South Carolina, Charleston, SC, USA

    Maurizio Del Poeta

Authors
  1. Arpita Singh
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  2. Asfia Qureshi
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  3. Maurizio Del Poeta
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Corresponding author

Correspondence to Maurizio Del Poeta .

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Editors and Affiliations

  1. Biochemisches Institut, Universität Zürich, Winterthurerstr. 190, Zürich, 8057, Switzerland

    Attila Becskei

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Singh, A., Qureshi, A., Del Poeta, M. (2011). Quantitation of Cellular Components in Cryptococcus neoformans for System Biology Analysis. In: Becskei, A. (eds) Yeast Genetic Networks. Methods in Molecular Biology, vol 734. Humana Press. https://doi.org/10.1007/978-1-61779-086-7_16

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  • DOI: https://doi.org/10.1007/978-1-61779-086-7_16

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-085-0

  • Online ISBN: 978-1-61779-086-7

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