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Immuno-Analysis and FACS Sorting of Adult Muscle Fiber-Associated Stem/Precursor Cells

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Book cover Protocols for Adult Stem Cells

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 621))

Abstract

We describe the immunostaining methods we commonly use to detect the more robust cell markers identifying the various cell populations obtained by the enzymatic and or mechanical dissociation of muscle satellite cells: CD34, m-Cadherin, and Pax7, self-renewing muscle stem cells expressing CXCR4 and β1-integrin, populations of proliferative myogenic progenitor cells expressing, Pax3, Pax7, Myf5, MyoD1, and desmin, differentiating myoblasts expressing myogenin and eMHC, and the CD45 expressing leukocyte lineage cells that infiltrate injured and regenerating skeletal muscle.

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References

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Acknowledgments

We thank the National Institutes of Health, National Institute on Aging (R01 AG027252 and K01 AR050515 to I.M.C.), the Stem Cell Research Foundation (Maryland, to I.M.C.), and the Harvard Stem Cell Institute (Cambridge, MA, to A.J.W.) for recent and current funding of these endeavors.

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Authors and Affiliations

  1. Department of BioEngineering, University of California, Berkeley, CA, USA

    Michael J. Conboy & Irina M. Conboy

  2. Section on Developmental and Stem Cell Biology and Department of Pathology, Joslin Diabetes Center and Harvard Stem Cell Institute, Boston, MA, USA

    Massimiliano Cerletti & Amy J. Wagers

Authors
  1. Michael J. Conboy
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  2. Massimiliano Cerletti
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  3. Amy J. Wagers
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  4. Irina M. Conboy
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Corresponding author

Correspondence to Michael J. Conboy .

Editor information

Editors and Affiliations

  1. Dept. Bioengineering, University of California, Berkeley, Evans Hall 479, Berkeley, 94720-1762, USA

    Irina M. Conboy

  2. Helen Wills Neuroscience Inst., University of California, Berkeley, Stanley Hall 176, Berkeley, 94720-3220, USA

    David V. Schaffer

  3. Lawrence Berkeley National Lab., Dept. Cancer Biology, University of California, Berkeley, Cyclotron Rd. 1, Berkeley, 94720, USA

    Mary Helen Barcellos-Hoff

  4. Dept. Bioengineering, University of California, Berkeley, Stanley Hall, Berkeley, 94720-1762, USA

    Song Li

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© 2010 Springer Science+Business Media, LLC

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Conboy, M.J., Cerletti, M., Wagers, A.J., Conboy, I.M. (2010). Immuno-Analysis and FACS Sorting of Adult Muscle Fiber-Associated Stem/Precursor Cells. In: Conboy, I., Schaffer, D., Barcellos-Hoff, M., Li, S. (eds) Protocols for Adult Stem Cells. Methods in Molecular Biology™, vol 621. Humana Press. https://doi.org/10.1007/978-1-60761-063-2_11

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  • DOI: https://doi.org/10.1007/978-1-60761-063-2_11

  • Published:

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-062-5

  • Online ISBN: 978-1-60761-063-2

  • eBook Packages: Springer Protocols

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