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Camptothecin Production by In Vitro Cultures and Plant Regeneration in Ophiorrhiza Species

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Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants

Part of the book series: Methods in Molecular Biology ((MIMB,volume 547))

Summary

Camptothecin derivatives are clinically used for the treatment of various human cancers. These derivatives are semi-synthesized from camptothecin which is isolated from the extracts of Camptotheca acuminata and Nothapodytes foetida. For the feasible production of camptothecin, the protocols for the tissue cultures of Ophiorrhiza species, O. pumila, O. liukiuensis and O. kuroiwai, have been established. The established aseptic plants and hairy roots produced camptothecin, and O. pumila hairy roots accumulated highest amount of camptothecin. Furthermore, we have established methods of plant regeneration from O. pumila hairy roots.

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Acknowledgments

This work was supported in part by Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (JSPS), and by CREST of the Japan Science and Technology Agency (JST).

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Authors and Affiliations

  1. Graduate School of Pharmaceutical Sciences, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba, 263-8522, Japan

    Kazuki Saito

Authors
  1. Takashi Asano
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  2. Hiroshi Sudo
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  3. Mami Yamazaki
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  4. Kazuki Saito
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Corresponding author

Correspondence to Kazuki Saito .

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Editors and Affiliations

  1. Department of Applied Biology, University of Helsinki, Helsinki, Finland

    S. Mohan Jain

  2. Department of Plant Agriculture, University of Guelph, Guelph, ON, Canada

    Praveen K. Saxena

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Asano, T., Sudo, H., Yamazaki, M., Saito, K. (2009). Camptothecin Production by In Vitro Cultures and Plant Regeneration in Ophiorrhiza Species. In: Jain, S.M., Saxena, P.K. (eds) Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants. Methods in Molecular Biology, vol 547. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-287-2_27

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  • DOI: https://doi.org/10.1007/978-1-60327-287-2_27

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-60327-286-5

  • Online ISBN: 978-1-60327-287-2

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