Abstract
The Myrtle (Myrtus communis L.) is an evergreen shrub typical of the Mediterranean area; it is an interesting plant with multipurpose use. The ornamental use takes into account the production of green cut branches for indoor decoration and production of pot plants for gardening. In this species, there is a great variability in the natural germplasm around the Mediterranean coasts for type and size of fruit, plant architecture, leaf size and internode length. Selected genotypes have been successfully sterilized and cultured in vitro. The shoots were multiplied on MS (16) salts and vitamins, with 0.5 mg/L BA and 0.2 mg/L IAA. Clones showed variation of multiplication rate and rooting percentage. IAA or IBA at 0.5 mg/L increased the rooting percentage and noticed differences in root number and length. The sucrose concentration can affect rooting, such as light intensity during the in vitro rooting phase can modulate biomass production and chlorophyll content. The combination of these factors enhanced the frequency rate of acclimatization.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Cervelli C, Ruffoni B, Castello S, Savona M (2001) Variability of vegetative traits with ornamental value in spontaneous Myrtus communis L. genotypes. Proc Convegno Biodiversità 2:135–144
Mulas M, Perinu B, Francescani AHD, Peana I (2001) Variability on fruit quality of different mirtle genotypes. Atti Convegno Biodiversità Bari 1:103–108
Bruna S, Portis E, Cervelli C, De Benedetti L, Schiva T, Mercuri A (2007) AFLP-based genetic relationships in the Mediterranean myrtle (Myrtus communis L.). Sci Hortic 113(4):370–375
Uhring J (1983) In vitro propagation of Sedum and Myrtus cultivars. HortScience 18(4):453–454
Khosh-Khui M, Shekafandeh A, Azarakhsh H (1984) Micropropagation of myrtle. Sci Hortic 22:133–137
Nobre J (1994) In vitro shoot proliferation of Myrtus communis L., from field grown plants. Sci Hortic 58:253–258
Ruffoni B, Gazzano A, Costantino C (1994) Myrtus communis micropropagation: indolacetic acid effect on rooting. Italus Hortus 1:8–12
Lucchesini M, Massai R, Gucci R (1998) Radicazione in vitro di Mirto (Myrtus communis L.) in contenitori permeabili agli scambi gassosi. Atti IV Giornate Scientifiche SOI, Sanremo 1–3 aprile 1:43–44
Lucchesini M, Mensuali-Sodi A, Serra G (2001) Propagazione in vitro e studio del microambiente in specie mediterranee. Flortecnica 4:66–68
Morini S, Freudiani F, D’Onofrio C (2002) Indagini sulla micropropagazione del mirto. Italus Hortus 9(2):41–48
Rigodi MP, Cani MR, Chessa G, Zurru R (2004) Prime valutazioni sulla risposta alla coltura in vitro di cloni di Myrtus communis L. Italus Hortus 11(4):315–317
Parra R, Amo-Marco JB (1996) Effect of plant growth regulators and basal media on in vitro shoot proliferation and rooting of Myrtus communis L. Biol Plant 38:161–168
Ruffoni B, Airò M, Fascella G, Mascarello C, Zizzo G, Cervelli C (2003) Propagation and acclimatization of ornamental Myrtle genotypes. Acta Hort 616:255–258
Jeon MW, Ali MB, Hahn EJ, Paek KY (2004) Photosynthetic pigments, morphology and leaf gas exchange durino ex vitro acclimatization of micropropagated CAM Doritaenopsis plantlets under relative humidity and air temperature. Environ Exp Bot 55:183–194
Mascarello C, Melis R, Mantovani E, Ruffoni B (2009) Evaluation of the role of light intensity and hormonal treatment during in vitro rooting of Myrtus micropropagated plants related to acclimatization performance. A&MP 2007 SYMP. Acta Hort 812.379–3
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:478–487
Lichtenthaler HK, Buschmann C (2001) Chlorophylls and carotenoids – Measurement and characterisation by UV-VIS. In: Current Protocols in Food Analytical Chemistry (CPFA), Suppl 1. John Wiley, New York, pp F4.3.1–F4.3.8
Acknowledgments
The results reported in this paper comes from research financed by the Italian Ministry of Agriculture, Fund “Programma di sviluppo per il Mezzogiorno d’Italia: ricerca ed innovazione tecnologica” (Project 205/7303/05 FRUMED “Valorizzazione delle produzioni florovivaistiche del meridione”); publication N.57.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2010 Humana Press, a part of Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Ruffoni, B., Mascarello, C., Savona, M. (2010). In Vitro Propagation of Ornamental Myrtus (Myrtus communis). In: Jain, S., Ochatt, S. (eds) Protocols for In Vitro Propagation of Ornamental Plants. Methods in Molecular Biology, vol 589. Humana Press. https://doi.org/10.1007/978-1-60327-114-1_24
Download citation
DOI: https://doi.org/10.1007/978-1-60327-114-1_24
Published:
Publisher Name: Humana Press
Print ISBN: 978-1-60327-390-9
Online ISBN: 978-1-60327-114-1
eBook Packages: Springer Protocols