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Protein Blotting and Detection pp 9–22Cite as

Introduction to Protein Blotting

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 536))

Summary

Protein blotting is a powerful and important procedure for the immunodetection of proteins following electrophoresis, particularly proteins that are of low abundance. Since the inception of the protocol for protein transfer from an electrophoresed gel to a membrane in 1979, protein blotting has evolved greatly. The scientific community is now confronted with a variety of ways and means to carry out this transfer.

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Authors and Affiliations

  1. Oklahoma Medical Research Foundation, 825 NE 13th Street, OK 73104, Oklahoma City, USA

    Biji T. Kurien & R. Hal Scofield

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  1. Biji T. Kurien
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  2. R. Hal Scofield
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Correspondence to Biji T. Kurien .

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Editors and Affiliations

  1. Oklahoma Medical Research Foundation, NE. 13th Street 825, Oklahoma City, 73104, U.S.A.

    Biji T. Kurien

  2. Oklahoma Medical Research Foundation, NE. 13th Street 825, Oklahoma City, 73104, U.S.A.

    R. Hal Scofield

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Kurien, B.T., Scofield, R.H. (2009). Introduction to Protein Blotting. In: Kurien, B., Scofield, R. (eds) Protein Blotting and Detection. Methods in Molecular Biology, vol 536. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-542-8_3

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  • DOI: https://doi.org/10.1007/978-1-59745-542-8_3

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-934115-73-2

  • Online ISBN: 978-1-59745-542-8

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