Abstract
Protocols for isolation of human hepatocytes have been developed. The isolated cells can be used not only in research but also for transplantation in patients with liver disease, especially acute liver failure and liverbased metabolic/synthetic conditions. The aim of hepatocyte transplantation is to correct the missing liver function(s) and allow either the recovery of the liver or buy the patient time until a suitable donor liver is available for transplantation.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Fisher, R. A., Strom, S. C. (2006) Human hepatocyte transplantation: worldwide results. Transplantation 82, 441–449.
Berry, M. S., Friend, D. S. (1965) High yield preparation of isolated rat liver parenchymal cells. J Cell Biol 43, 506–520.
Seglen, P. O. (1976) Preparation of rat liver cells. Meth Cell Biol 13, 29–83.
Strom, S. C., Dorko, K., Thompson, M. T., etal. (1998) Large scale isolation and culture of human hepatocytes, in (Franco, D., Boudjema, K., Varet, B., eds.), Îlots de Langerhans et hépatocytes, pp. 195-205. Les Editions INSERM, Paris.
Mitry, R. R., Hughes, R. D., Aw, M. M., et al. (2003) Human hepatocyte isolation and relationship of cell viability to early graft function. Cell Transpl 12, 69–74.
Freshney, R. I. (2000) Culture of Animal Cells. Wiley-Liss, New York, NY, pp. 309–328.
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2009 Humana Press, a part of Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Mitry, R. (2009). Isolation of Human Hepatocytes. In: Dhawan, A., Hughes, R. (eds) Hepatocyte Transplantation. Methods in Molecular Biology, vol 481. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-201-4_2
Download citation
DOI: https://doi.org/10.1007/978-1-59745-201-4_2
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-60761-172-1
Online ISBN: 978-1-59745-201-4
eBook Packages: Springer Protocols