Abstract
The isolation and experimental manipulation of multipotent precursors is of increasing therapeutic relevance. We recently reported the generation of cultures of Skin-derived Precursors (‘SKPs’), multipotent cells that can be isolated from the dermis of embryonic, neonatal, and adult rodent skin (1), and from adult human skin (2) SKPs have similarities to stem cells of the embryonic neural crest (3), and differentiate into a variety of neural and mesodermal cell phenotypes, including peripheral neurons and glial cells, smooth muscle cells, bone, cartilage, and adipocytes (3–5). Here, we detail the establishment, propagation, neural differentiation, and immunocytochemical analysis of SKP cultures.
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References
Toma, J.G., Akhavan, M., Fernandes, K.J., Barnabe-Heider, F., Sadikot, A., Kaplan, D.R., and Miller, F.D., Isolation of multipotent adult stem cells from the dermis of mammalian skin. Nat Cell Biol, 2001. 3(9): 778–84.
Toma, J.G., McKenzie, I.A., Bagli, D., and Miller, F.D., Isolation and characterization of multipotent skin-derived precursors from human skin. Stem Cells, 2005. 23(6): 727–37.
Fernandes, K.J., McKenzie, I.A., Mill, P., Smith, K.M., Akhavan, M., Barnabe-Heider, F., Biernaskie, J., Junek, A., Kobayashi, N.R., Toma, J.G., Kaplan, D.R., Labosky, P.A., Rafuse, V., Hui, C.C., and Miller, F.D., A dermal niche for multipotent adult skin-derived precursor cells. Nat Cell Biol, 2004. 6(11): 1082–93.
Fernandes, K.J., Kobayshi, N.R., Gallagher, C.J., Barnabe-Heider, F., Aumont, A., Kaplan, D.R., and Miller, F.D., Analysis of the neurogenic potential of multipotent Skin-derived Precursors. Exp Neurol, 2006. 201(1): 32–48.
McKenzie, I.A., Biernaskie, J., Toma, J.G., Midha, R., and Miller, F.D., Skin-derived precursors generate myelinating Schwann cells for the injured and dysmyelinated nervous system. J Neurosci, 2006. 26(24): 6651–60.
Le Douarin, N.M. and Kalcheim, C., The Neural Crest, edn 2. 1999, Cambridge, UK: Cambridge University Press.
Barembaum, M. and Bronner-Fraser, M., Early steps in neural crest specification. Semin Cell Dev Biol, 2005. 16(6): 642–6.
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Fernandes, K.J., Miller, F.D. (2009). Isolation, Expansion, and Differentiation of Mouse Skin-Derived Precursors. In: Audet, J., Stanford, W.L. (eds) Stem Cells in Regenerative Medicine. Methods in Molecular Biology, vol 482. Humana Press. https://doi.org/10.1007/978-1-59745-060-7_10
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DOI: https://doi.org/10.1007/978-1-59745-060-7_10
Publisher Name: Humana Press
Print ISBN: 978-1-58829-797-6
Online ISBN: 978-1-59745-060-7
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