Skip to main content
SpringerLink
Log in

Protein Electrophoresis

  • Protocol
Book cover Molecular Biomethods Handbook

Part of the book series: Springer Protocols Handbooks ((SPH))

Abstract

There are an estimated 100,000 genes in the entire human genome, 10% of which may be expressed in a particular cell-type at a given time Purlfying and analyzing the products of genes (proteins), from humans and other organisms, offers a unique challenge to the blochemlst and one that is essential to our understanding of blologlcal function The diversity in size, lomc charge, solublhty, stability, cellular expression, and posttranslatlonal modlficatlon of proteins requires protein-analysis techniques that have high-resolving capacities Electrophoretlc separation techniques provide a hlghresolution, adaptable and simple range of methodologies used in nearly all forms of protein analysis (1)

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Dunn, M. J. (1993) Gel Elecfrophoresis: Proteins. Bios, Oxford, UK.

    Google Scholar 

  2. Estep, T. N. and Miller, T. J. (1986) Optimisation of erythrocyte membrane glycoprotein fluorescent labelling with dansylhydrazine after polyacrylarnide gel electrophoresis. Anal.Biochem. 157, 100–105.

    Article  PubMed  CAS  Google Scholar 

  3. Debruyne, I. (1983) Staining of alkali-labile phophoproteins and alkaline phosphates on polyacrylamide gels. Anal. Biochem. 133, 110–115.

    Article  PubMed  CAS  Google Scholar 

  4. Tsai, C.M. and Frasch, C. E. (1982) A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal. Biochem. 119, 115–119.

    Article  PubMed  CAS  Google Scholar 

  5. Bag-Hansen, T C (1993) Immunoelectrophoresls, in Gel Electrophoreszs of Protezns (Hames, B D and Rickwood, D, eds), IRL, Oxford, UK, pp 273–300

    Google Scholar 

  6. Raymond, S and Wemtraud, L S (1959) Acrylamlde gel as a supporting medmm for zone electrophoresls Sczence 130,711–721

    Article  CAS  Google Scholar 

  7. O’Connel, P B H and Brady, C J (1976) Polyacrylamlde gels with modified crosslinkages Anal Blochem 76,63–73

    Article  Google Scholar 

  8. Rodbard, D, Chrambach, A, and Wels, G H (1974) in Electrophorests and Zsoelectrzc Focuszng zn Polyacrylamzde Gel (Allen, R C and Mauvrer, H R, eds), Walter de Grugtner, Berlm, p 62

    Google Scholar 

  9. Heussen, C and Dowdle, E B (1980) Electrophorectlc analysis of plasmmogen actlvtators in polyacrylamlde gel containing sodium dodecyl sulphate and copolymerlsed substrates Anal Bzoche 102, 196–202

    Article  CAS  Google Scholar 

  10. Gabriel, O (1971) Locating enzymes on gels Methods Enzymol 22,578–604

    Article  Google Scholar 

  11. Dunn, M J (1993) Electrophoresls under native condltlons, in Gel Electrophoreszs Protezns, BIOS, Oxford, UK, pp 31–39

    Google Scholar 

  12. Carr-Eusbe, D, Lederer, F, DlCp, K H, and Elsevler, S in (1991) Processing of the precursor of protamme P2 in mouse Blochem J 277,39–45

    Google Scholar 

  13. Rlghettl, P G (1983) Isoelectric Focuszng Theory Methodology and Applzcatzons Elsevler, Amsterdam

    Google Scholar 

  14. Weber, K and Osborn, M (1969) The rellablhty of molecular weight determmatlon by dodecyl sulphate polyacrylamlde gel electrophoresls J BtoZ Chem 244,4406–4412

    CAS  Google Scholar 

  15. Laemmh, U, K (1970) Cleavage of structural proteins during the assembly of the head of the bacterlopahge T4 Nature 227,680–685

    Article  Google Scholar 

  16. Schagger, H and von Jagow, G (1987) Tncme-sodium dodecyl sulfate-polyacrylamlde gel electrophoresls for the separation of proteins in the range from 1 to 100 KDa Anal Blochem 166,368–379

    Article  CAS  Google Scholar 

  17. Poduslo, J F and Rodbard, D (1982) Molecular weight estimation using sodmm dodecyl sulphate-pore gradient electrophoresls Anal Blochem 101,394–406

    Article  Google Scholar 

  18. Wilson, C in (1983) Staming of proteins on gels comparisons of dyes and procedures Methods Enzymol 91,236–246

    Article  PubMed  CAS  Google Scholar 

  19. Neuhoff, V, Arold, N, Taube, D, and Ehrhardt, W (1988) Improved staming of proteins in polyacrylamlde gels mcluding lsoelectrlc focusing gels with clear background and nanogram sensltlvlty using Coomassle brllllant blue G-250 and R-250 Electrophoreszs 9,255–262

    Article  CAS  Google Scholar 

  20. Ward, A H and Allen, W S (1972) An improved procedure for protein staming in polyacrylamlde gels with a new type of Coomassle bnlhant blue Anal Bzochem 48,617–620

    Article  Google Scholar 

  21. Oakley, B R, Klrsch, D R, and Morris, N R (1980) A slmphfied ultrasensItIve sliver stain for detecting protems in polyacrylamlde gels Anal Bzochem 105,361–363

    Article  CAS  Google Scholar 

  22. Bonner, W in and Laskey, R A (1974) A film detectton method for tntmm-labelled protems and nucleic actds in polyacrylamlde gels Eur J Blochem 46, 83–88

    CAS  Google Scholar 

  23. Cleveland, D W, Fischer, S G, Klrschner, M W, and Laemmh, U K (1977) Peptlde mapping by limited proteolysls in sodium dodecyl sulfate and analysis by gel electro-1 phoresls J Bzol Chem 252,1102–1106

    CAS  Google Scholar 

  24. Gultekm, H and Heermann, K H (1988) The use of polyvmyhdenedlfluorlde membranes as a general blotting matrix Anal Bzochem 172, 320–329

    Article  Google Scholar 

  25. Kennedy, T E, Wager-Smith, K, Barzllal, A., Kandel, E R, and Sweatt, J D (1988)Sequencing proteins from acrylamme gels Nature 336,499,500

    Article  PubMed  CAS  Google Scholar 

  26. Pluskal, M G, Przekop, M B, Kavoman, M R, Vecoh, C, and Hicks, D A (1986)ImmobllonTM PVDF transfer membrane a new membrane substrate for Western blotting of proteins BloTechntques 4,272–283

    CAS  Google Scholar 

  27. Hochstrasser, D F, Harrington, M G, Hochstrasser, A-C, Miller, M J, and Menl, C R (1988) Methods for increasing the resolution of two-dlmenslonal protem electrophore-SISAnal Blochem 173,424–435

    Article  CAS  Google Scholar 

  28. Perdew, G H, Scaup, H, and Sehvonchlck, D P (1983) The use of detergents in twodimensIona gel electrophoresls of trout-lrver mlcrosomes Anal Bzochem 135,453–455

    Article  CAS  Google Scholar 

  29. Gorg, A (1993) Two-dImensiona electrophoresls with lmmoblhzed pH gradients current state Bzochem Sot Trans 21, 130–133

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Leicester Unzverszty, Leicester, UK

    Paul Richards

Authors
  1. Paul Richards
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. University of Herfordshire, Hatfield, UK

    Ralph Rapley  & John M. Walker  & 

Rights and permissions

Reprints and permissions

Copyright information

© 1998 Humana Press Inc , Totowa, NJ.

About this protocol

Cite this protocol

Richards, P. (1998). Protein Electrophoresis. In: Rapley, R., Walker, J.M. (eds) Molecular Biomethods Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-59259-642-3_32

Download citation

  • DOI: https://doi.org/10.1007/978-1-59259-642-3_32

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-501-0

  • Online ISBN: 978-1-59259-642-3

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation