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Chimera Research pp 205–220Cite as

Isolation, Cryopreservation, and Transplantation of Spermatogonial Stem Cells

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2005))

Abstract

Spermatogonial stem cell (SSC) culture and transplantation pave the way for clinical restoration of fertility in male prepubertal cancer survivors. In this chapter we detail the steps for isolating and freezing testicular tissue along with protocols for the subsequent recovery from cryopreservation and transplantation of cells into a recipient testis. Transplantation of cultured or thawed SSCs provides not only a functional assay for identification of stem cells, a critical tool for the study of the germline stem cell niche in model organisms, but also a framework for reconstitution of spermatogenesis in humans. As proof of concept, the outlined methods have been performed successfully in the murine model and have the potential to be translated to clinical environments.

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Acknowledgments

We thank James Hayden, RBP, FBCA, for assistance in preparation of figures and Mary Avarbock for advice and expert assistance. This work was supported by the National Institute of Child Health and Human Development (R.L.B.) and the Robert J. Kleberg, Jr. and Helen C. Kleberg Foundation (R.L.B.).

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Authors and Affiliations

  1. Department of Biomedical Sciences, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, USA

    Nilam Sinha, Eoin C. Whelan & Ralph L. Brinster

Authors
  1. Nilam Sinha
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  2. Eoin C. Whelan
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  3. Ralph L. Brinster
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Corresponding author

Correspondence to Eoin C. Whelan .

Editor information

Editors and Affiliations

  1. Department of Bioethics, Case Western Reserve University, School of Medicine, Cleveland, OH, USA

    Insoo Hyun

  2. Department of Psychiatry, Yale University School of Medicine, New Haven, CT, USA

    Alejandro De Los Angeles

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Sinha, N., Whelan, E.C., Brinster, R.L. (2019). Isolation, Cryopreservation, and Transplantation of Spermatogonial Stem Cells. In: Hyun, I., De Los Angeles, A. (eds) Chimera Research . Methods in Molecular Biology, vol 2005. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9524-0_14

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  • DOI: https://doi.org/10.1007/978-1-4939-9524-0_14

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9523-3

  • Online ISBN: 978-1-4939-9524-0

  • eBook Packages: Springer Protocols

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