Abstract
Naïve T cells are in a quiescent state under homeostasis but respond to antigen stimulation by exiting from quiescence and entering the cell cycle. Appropriate regulation of quiescence is crucial for maintaining T cell homeostasis at steady state and initiating proper T cell responses to antigen stimulation. Emerging evidence indicates that signaling by mechanistic target of rapamycin (mTOR) plays a central role in the control of T cell quiescence and antigen-induced exit from quiescence through coordinating immune signals, cellular metabolic programs, and cell cycle machinery. The mTOR-dependent regulation of quiescence has also been implicated in the differentiation and function of memory T cells. In this chapter, we describe techniques to assess quiescent state of naïve T cells under steady state and exit from quiescence upon TCR stimulation.
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Acknowledgments
The authors acknowledge H. Zeng and Y. Wang for scientific inputs and editing. This work was supported by NIH AI105887, AI101407, CA176624 and NS064599, and American Asthma Foundation.
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Yang, K., Chi, H. (2018). Investigating Cellular Quiescence of T Lymphocytes and Antigen-Induced Exit from Quiescence. In: Lacorazza, H. (eds) Cellular Quiescence. Methods in Molecular Biology, vol 1686. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7371-2_12
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DOI: https://doi.org/10.1007/978-1-4939-7371-2_12
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