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Carbohydrate Depolymerization by Intricate Cellulosomal Systems

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Book cover Protein-Carbohydrate Interactions

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1588))

Abstract

Cellulosomes are multi-enzymatic nanomachines that have been fine-tuned through evolution to efficiently deconstruct plant biomass. Integration of cellulosomal components occurs via highly ordered protein–protein interactions between the various enzyme-borne dockerin modules and the multiple copies of the cohesin modules located on the scaffoldin subunit. Recently, designer cellulosome technology has been established to provide insights into the architectural role of catalytic (enzymatic) and structural (scaffoldin) cellulosomal constituents for the efficient degradation of plant cell wall polysaccharides. Owing to advances in genomics and proteomics, highly structured cellulosome complexes have recently been unraveled, and the information gained has inspired the development of designer cellulosome technology to new levels of complex organization. These higher-order designer cellulosomes have in turn fostered our capacity to enhance the catalytic potential of artificial cellulolytic complexes. In this chapter, methods to produce and employ such intricate cellulosomal complexes are reported.

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Author information

Authors and Affiliations

  1. Faculty of Biochemistry, Department of Biomolecular Sciences, The Weizmann Institute of Science, Ullmann Building of Life Sciences, Room 226, Rehovot, 76100, Israel

    Johanna Stern, Lior Artzi, Sarah Moraïs & Edward A. Bayer

  2. CIISA – Faculdade de Medicina Veterinária, Universidade de Lisboa, Avenida da Universidade Técnica, 1300-477, Lisbon, Portugal

    Carlos M. G. A. Fontes

Authors
  1. Johanna Stern
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  2. Lior Artzi
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  3. Sarah Moraïs
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  4. Carlos M. G. A. Fontes
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  5. Edward A. Bayer
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Corresponding author

Correspondence to Edward A. Bayer .

Editor information

Editors and Affiliations

  1. Agriculture and Agri-Food Canada, Lethbridge, Alberta, Canada

    D. Wade Abbott

  2. Biotechnology Institute, University of Groningen, Groningen, The Netherlands

    Alicia Lammerts van Bueren

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Stern, J., Artzi, L., Moraïs, S., Fontes, C.M.G.A., Bayer, E.A. (2017). Carbohydrate Depolymerization by Intricate Cellulosomal Systems. In: Abbott, D., Lammerts van Bueren, A. (eds) Protein-Carbohydrate Interactions. Methods in Molecular Biology, vol 1588. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6899-2_8

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  • DOI: https://doi.org/10.1007/978-1-4939-6899-2_8

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6898-5

  • Online ISBN: 978-1-4939-6899-2

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