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High Throughput Screening pp 143–157Cite as

Protein Kinase Selectivity Profiling Using Microfluid Mobility Shift Assays

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1439))

Abstract

Biochemical selectivity profiling is an integral part of early drug development. Typically compounds from optimization phase are regularly tested for off-target activities within or across target families. This article presents workflow and critical aspects of biochemical protein kinase profiling based on microfluidic mobility shift assays.

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Acknowledgment

I would like to thank Shin Numao and Patrik Roethlisberger for their helpful input to the manuscript. I would like to thank Joerg Trappe for suggesting and encouraging the drafting of this manuscript.

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Authors and Affiliations

  1. CPC Screening Sciences, Novartis Pharma AG, Novartis Institutes for Biomedical Research, 4002, Basel, Switzerland

    Peter Drueckes

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  1. Peter Drueckes
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Correspondence to Peter Drueckes .

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Editors and Affiliations

  1. Epizyme, Inc., Cambridge, Massachusetts, USA

    William P. Janzen

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Drueckes, P. (2016). Protein Kinase Selectivity Profiling Using Microfluid Mobility Shift Assays. In: Janzen, W. (eds) High Throughput Screening. Methods in Molecular Biology, vol 1439. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3673-1_9

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  • DOI: https://doi.org/10.1007/978-1-4939-3673-1_9

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3671-7

  • Online ISBN: 978-1-4939-3673-1

  • eBook Packages: Springer Protocols

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