Abstract
With the ability to obtain tens of millions of reads, high-throughput messenger RNA sequencing (RNA-Seq) data offers the possibility of estimating abundance of isoforms and finding novel transcripts. In this chapter, we describe a protocol to construct an RNA-Seq library for sequencing on Illumina NGS platforms, and a computational pipeline to perform RNA-Seq data analysis. The protocols described in this chapter can be applied to the analysis of differential gene expression in control versus 17β-estradiol treatment of in vivo or in vitro systems.
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Acknowledgments
We thank Dr. Thomas Girke at the University of California Riverside for sharing his R scripts.
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Liang, H., Zeng, E. (2016). RNA-Seq Experiment and Data Analysis. In: Eyster, K.M. (eds) Estrogen Receptors. Methods in Molecular Biology, vol 1366. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3127-9_9
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DOI: https://doi.org/10.1007/978-1-4939-3127-9_9
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3126-2
Online ISBN: 978-1-4939-3127-9
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