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Plant Isoprenoids pp 213–226Cite as

Prenylquinone Profiling in Whole Leaves and Chloroplast Subfractions

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1153))

Abstract

Prenylquinones are indispensable molecules in plants and animals. In plants, phylloquinone (vitamin K) and plastoquinone are electron carriers during photosynthesis in chloroplasts, whereas tocopherol (vitamin E) functions as a lipid antioxidant. The biosynthetic pathways of the prenylquinones have been largely characterized but the mechanisms regulating their production and distribution in various subcompartments of the chloroplast are only starting to emerge. Research on chloroplast lipid droplets (plastoglobules) has unraveled a complex network of intersecting prenylquinone metabolic pathways that are providing unprecedented insight into the regulatory processes. In this chapter, we describe how to isolate chloroplast membrane fractions, in particular the plastoglobule lipid droplets, and how to profile the prenylquinones that are contained in these fractions.

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Author information

Authors and Affiliations

  1. Laboratory of Plant Physiology, University of Neuchâtel, Neuchâtel, Switzerland

    Felix Kessler

  2. Chemical Analytical Service of the Swiss Plant Science Web, University of Neuchâtel, Rue Emile-Argand 11, CH-2009, Neuchâtel, Switzerland

    Gaetan Glauser

Authors
  1. Felix Kessler
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  2. Gaetan Glauser
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Corresponding author

Correspondence to Gaetan Glauser .

Editor information

Editors and Affiliations

  1. Centre for Research in Agricultural Genomic (CRAG), CSIC-IRTA-UAB-UB, Barcelona, Spain

    Manuel Rodríguez-Concepción

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Kessler, F., Glauser, G. (2014). Prenylquinone Profiling in Whole Leaves and Chloroplast Subfractions. In: Rodríguez-Concepción, M. (eds) Plant Isoprenoids. Methods in Molecular Biology, vol 1153. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0606-2_15

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  • DOI: https://doi.org/10.1007/978-1-4939-0606-2_15

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0605-5

  • Online ISBN: 978-1-4939-0606-2

  • eBook Packages: Springer Protocols

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