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Biofilm Formation in the 96-Well Microtiter Plate

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Pseudomonas Methods and Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1149))

Abstract

The microtiter plate (also called 96-well plate) assay for studying biofilm formation is a method which allows for the observation of bacterial adherence to an abiotic surface. In this assay, bacteria are incubated in vinyl “U”-bottom or other types of 96-well microtiter plates. Following incubation, planktonic bacteria are rinsed away, and the remaining adherent bacteria (biofilms) are stained with crystal violet dye, thus allowing visualization of the biofilm. If quantitation is desired, the stained biofilms are solubilized and transferred to a 96-well optically clear flat-bottom plate for measurement by spectrophotometry.

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Acknowledgements

This work is supported by RSFG (Indiana University Purdue University Indianapolis) and PRF (Purdue University) to Gregory G. Anderson.

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Authors and Affiliations

  1. Department of Biology, Indiana University Purdue University Indianapolis, 723 West Michigan Street, SL 320, Indianapolis, IN, 46202, USA

    Barbara M. Coffey & Gregory G. Anderson

Authors
  1. Barbara M. Coffey
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  2. Gregory G. Anderson
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Corresponding author

Correspondence to Gregory G. Anderson .

Editor information

Editors and Affiliations

  1. MRC Centre for Molecular Bacteriology and Infection (CMBI) Department of Life Sciences, Imperial College, London, UK

    Alain Filloux

  2. Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, Granada, Spain

    Juan-Luis Ramos

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Coffey, B.M., Anderson, G.G. (2014). Biofilm Formation in the 96-Well Microtiter Plate. In: Filloux, A., Ramos, JL. (eds) Pseudomonas Methods and Protocols. Methods in Molecular Biology, vol 1149. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-0473-0_48

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  • DOI: https://doi.org/10.1007/978-1-4939-0473-0_48

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-0472-3

  • Online ISBN: 978-1-4939-0473-0

  • eBook Packages: Springer Protocols

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