Abstract
This chapter reviews the proteomics research performed to elucidate the molecular processes associated with embryo initiation and development in Brassica napus (rapeseed) microspore-derived embryo cultures. Classical biochemical methods such as combined [35S]-methionine labelling and 2-D gel electrophoresis (2-DE) have been used for the large-scale study of proteins differentially expressed during the inductive phase of embryogenesis. Immuno-cytological experiments have been carried out to study the (sub)cellular localization of putative marker proteins for embryo induction. Changes in post-translational modification of proteins (phosphorylation) have been detected during the induction phase, as have changes in the profile of proteins en peptides secreted by developing embryos. We also discuss recent developments in quantitative proteomics technologies such as fluorescence labelling of protein samples and stable isotope labelling by amino acids in cell cultures that enable more detailed expression analyses and the identification of low abundant proteins in complex mixtures.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Boutilier K, Fiers M, Liu CM, van der Geest AHM (2005) Biochemical and molecular aspects of haploid embryogenesis. In: Palmer CE, Keller WA, Kasha KJ, (eds) Haploids in Crop Improvement II, Vo l 56. Springer, Heidelberg, pp 73–95
Binarova P, Straatman K, Hause B, Hause G, Van Lammeren AAM (1993) Nuclear DNA synthesis during the induction of embryogenesis in cultured microspores and pollen of Brassica napus L. Theor Appl Genet 87:9–16
Binarova P, Hause G, Cenklova V, Cordewener JHG, Van Lookeren Campagne MM (1997) A short severe heat shock is required to induce embryogenesis in late bicellular pollen of Brassica napus L. Sex Plant Reprod 10:200–208
Cassoni S, Spencer M, Walker K, Lindsey K (2005) Laser capture microdissection for the analysis of gene expression during embryogenesis of Arabidopsis. Plant J 42:111–123
Cordewener JHG, Busink R, Traas JA, Custers JBM, Dons JJM, Van Lookeren Campagne MM (1994) Induction of microspore embryogenesis in Brassica napus is accompanied by specific changes in protein synthesis. Planta 195:50–56
Cordewener JHG, Hause G, Görgen E, Busink R, Hause B, Dons JJM, Van Lammeren AAM, Van Lookeren Campagne MM, Pechan P (1995) Changes in synthesis and localization of the 70 kDa class of heat shock proteins accompany the induction of embryogenesis in Brassica napus L. microspores. Planta 196:747–755
Cordewener JHG, Bergervoet J, Liu CM (2000) Changes in protein synthesis and phosphorylation during microspore embryogenesis in Brassica napus. J Plant Physiol 156:156–163
Custers JBM, Cordewener JHG, Nöllen Y, Dons JJM, Van Lookeren Campagne MM (1994) Temperature controls both gametophytic and sporophytic development in microspore cultures of Brassica napus. Plant Cell Rep 13:267–271
Fiers M (2007) Peptide signaling in plant development: functional analysis of CLE ligands in Arabidopsis. PhD Thesis, Wageningen University, The Netherlands
Gapper C, Dolan L (2006) Control of plant development by reactive oxygen species. Plant Physiol 141:341–345
Hari V (1980) Effect of cell density changes and conditioned media on carrot cell embryogenesis. Z Pflanzenphysiol 96:227–231
Hause G, Cordewener JHG, Ehrmanova M, Hause B, Binarova P, Van Lookeren Campagne MM, Van Lammeren AAM (1995) Cell cycle dependent distribution of phosphorylated proteins in microspores and pollen of Brassica napus L. detected by the monoclonal antibody MPM-2. Protoplasma 187:117–126
Huang B, Bird S, Kemble R, Simmonds D, Keller W, Miki B (1990) Effects of culture density, conditioned medium and feeder cultures on microspore embryogenesis in Brassica napus L. cv. Topas. Plant Cell Rep 8:594–597
Joosen R, Cordewener J, Supena EDJ, Vorst O, Lammers M, Maliepaard C, Zeilmaker T, Miki B, America T, Custers J, Boutilier K (2007) Combined transcriptome and proteome analysis identifies pathways and markers associated with the establishment of rapeseed microspore-derived embryo development. Plant Physiol 144:155–172
Mittler R, Vanderauwera S, Gollery M, Van Breusegem F (2004) Reactive oxygen gene network of plants. Trends Plant Sci 9:490–498
Pechan PM and Keller WA (1988) Identification of potentially embryogenic microspores in Brassica napus. Physiol Plant 74:377–384
Pechan PM, Bartels D, Brown DCW, Schell J (1991) Messenger-RNA and protein changes associated with induction of Brassica microspore embryogenesis. Planta 184:161–165
Shariatpanahi ME, Bal U, Heberle-Bors E, Touraev A (2006) Stresses applied for the re-programming of plant microspores towards in vitro embryogenesis. Physiol Plant 127:519–534
Tsuwamoto R, Fukuoka H, Takahata Y (2007) Identification and characterization of genes expressed in early embryogenesis from microspores of Brassica napus. Planta 225:641–652
von Eggeling F, Melle C, Ernst G (2007) Microdissecting the proteome. Proteomics 7:2729–2737
Zhao J, Newcomb W, Simmonds D (2003) Heat-shock proteins 70 kDa and 19 kDa are not required for induction of embryogenesis of Brassica napus L. cv. Topas microspores. Plant Cell Physiol 44:1417–1421
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2009 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Cordewener, J., van der Wal, F., Joosen, R., Boutilier, K., America, T. (2009). Proteomics in Rapeseed Microspore Embryogenesis. In: Touraev, A., Forster, B.P., Jain, S.M. (eds) Advances in Haploid Production in Higher Plants. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-8854-4_10
Download citation
DOI: https://doi.org/10.1007/978-1-4020-8854-4_10
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-8853-7
Online ISBN: 978-1-4020-8854-4
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)