Glutamine synthetase (GS) catalyzes the addition of ammonium to glutamic acid to form glutamine and plays a crucial role in the nitrogen assimilation of the sea anemone Aiptasia pallida and its endosymbiotic algae. We describe the cDNA cloning and sequence analysis of GS mRNA from A. pallida based on polymerase chain reaction (PCR) technology that employed a combination of degenerate and A. pallida specific primers. The sequenced cDNA approximates 1620 nucleotides and is characterized by an open reading frame of 1107 nucleotides that encodes a protein of 369 amino acid residues. Comparisons of the deduced sea anemone GS protein to a wide range of species demonstrated greatest amino acid sequence identity to sea urchin GS (66%) and least identity to green algae GS (51%). The sequenced cDNA can be used in future research to study GS gene expression in A. pallida.
Key words: algal-invertebrate symbiosis, Cnidaria, mRNA, nitrogen assimilation, PCR
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2004 Kluwer Academic Publishers
About this paper
Cite this paper
Smith, O.P., Marinov, A.D., Chan, K.M., Ferrier, M.D. (2004). Cloning and sequencing of cDNA encoding glutamine synthetase from the sea anemone Aiptasia pallida. In: Fautin, D.G., Westfall, J.A., Cartwrigh, P., Daly, M., Wyttenbach, C.R. (eds) Coelenterate Biology 2003. Developments in Hydrobiology, vol 178. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-2762-8_31
Download citation
DOI: https://doi.org/10.1007/978-1-4020-2762-8_31
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-4020-2761-1
Online ISBN: 978-1-4020-2762-8
eBook Packages: Springer Book Archive