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Automated Co-in Situ Hybridization and Immunofluorescence Using Archival Tumor Tissue

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In Situ Hybridization Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2148))

Abstract

In situ hybridization (ISH) and immunohistochemistry (IHC) are valuable tools for molecular pathology and cancer research. Recent advances in multiplex technology, assay automation, and digital image analysis have enabled the development of co-ISH IHC or immunofluorescence (IF) methods, which allow researchers to simultaneously view and quantify expression of mRNA and protein within the preserved tissue spatial context. These data are vital to the study of the control of gene expression in the complex tumor microenvironment.

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Author information

Authors and Affiliations

  1. MRC Toxicology Unit, University of Cambridge, Cambridge, UK

    Leah K. Officer, Kleopatra E. Andreou, Ana V. Teodósio, Zhangyi He & John P. Le Quesne

  2. Leicester Cancer Research Centre, University of Leicester, Leicester, UK

    John P. Le Quesne

  3. Leicester University Hospitals NHS Trust, University of Leicester, Leicester, UK

    John P. Le Quesne

Authors
  1. Leah K. Officer
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  2. Kleopatra E. Andreou
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  3. Ana V. Teodósio
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  4. Zhangyi He
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  5. John P. Le Quesne
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Corresponding author

Correspondence to John P. Le Quesne .

Editor information

Editors and Affiliations

  1. Molecular Histology, Bioneer A/S, Horsholm, Denmark

    Boye Schnack Nielsen

  2. Cancer Research UK, University of Cambridge, Cambridge, UK

    Julia Jones

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Officer, L.K., Andreou, K.E., Teodósio, A.V., He, Z., Le Quesne, J.P. (2020). Automated Co-in Situ Hybridization and Immunofluorescence Using Archival Tumor Tissue. In: Nielsen, B.S., Jones, J. (eds) In Situ Hybridization Protocols . Methods in Molecular Biology, vol 2148. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0623-0_16

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  • DOI: https://doi.org/10.1007/978-1-0716-0623-0_16

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0622-3

  • Online ISBN: 978-1-0716-0623-0

  • eBook Packages: Springer Protocols

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