Abstract
Different oligo-DNA surface functionalized polyorganosiloxane nanoparticles have been synthesized and characterized. The core–shell polyorganosiloxane nanoparticles possess a hydrodynamic radius of 14.1 (sample code CS1) and 12.1 nm (sample code CS2), respectively. Coupling of different short chain poly(ethylene oxide)s resulted in water soluble nanospheres. For CS2-PEO110, a number of approximately 500 PEO per siloxane nanosphere has been determined. Two different (partially fluorescently labelled) oligo-DNA sequences (12 and 20 bases, respectively) have been coupled to the PEO grafted on the nanoparticles with high coupling efficiency. MDCKII cells were incubated using a solution of these bio-hybrid nanoparticles and the successful intracellular uptake was visualized by fluorescence microscopy.
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References
Jungmann N, Schmidt M, Maskos M, Weis J, Ebenhoch J (2002) Macromolecules 35:6851
Jungmann N, Schmidt M, Maskos M (2001) Macromolecules 34:8347
Jungmann N, Schmidt M, Ebenhoch J, Weis J, Maskos M (2003) Angew Chem Int Ed 42:1714
Baumann F, Schmidt M, Deubzer B, Geck M, Dauth J (1994) Macromolecules 27:6102
Baumann F, Deubzer B, Geck M, Dauth J, Schmidt M (1997) Macromolecules 30:7568
Roos C, Schmidt M, Ebenhoch J, Baumann F, Deubzer B, Weis J (1999) Adv Mater 11:761
Emmerich O, Hugenberg N, Schmidt M, Sheiko S, Baumann F, Deubzer B, Weis J, Ebenhoch J (1999) Adv Mater 11:1299
Jungmann N, Schmidt M, Maskos M (2003) Macromolecules 36:3974
Graf C, Schärtl W, Maskos M, Schmidt M (2000) J Chem Phys 112:3031
Kramer T, Scholz S, Maskos M, Huber K (2004) J Colloid Interface Sci 279:447
Zimm B (1948) J Chem Phys 16:1093
Becker A, Köhler W, Müller B (1995) Ber Bunsenges Phys Chem 99:600
Lide DR (2003) CRC Handbook of Chemistry and Physics, 84th ed. CRC Press Inc., Boca Raton
Walsh MK, Wang X, Weimer BC (2001) J Biochem Biophys Methods 47:221
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© 2008 Springer-Verlag Berlin Heidelberg
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Diehl, C., Fluegel, S., Fischer, K., Maskos, M. (2008). Oligo-DNA Functionalized Polyorganosiloxane Nanoparticles. In: Auernhammer, G.K., Butt, HJ., Vollmer, D. (eds) Surface and Interfacial Forces – From Fundamentals to Applications. Progress in Colloid and Polymer Science, vol 134. Springer, Berlin, Heidelberg. https://doi.org/10.1007/2882_2008_086
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DOI: https://doi.org/10.1007/2882_2008_086
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