Regular ArticleTrypanosoma brucei: Inheritance of Kinetoplast DNA Maxicircles in a Genetic Cross and Their Segregation during Vegetative Growth
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The sexual side of parasitic protists
2021, Molecular and Biochemical ParasitologyCitation Excerpt :Many details of the mechanism of sexual reproduction in kinetoplastids remain to be worked out, including gamete fusion and the nature of the zygote. That cell fusion was involved in genetic exchange was deduced early on from the biparental inheritance of mitochondrial DNA in hybrid trypanosomes [60–62], but this by itself is improbable and contentious given what is known of the structure and replication of the mitochondrial DNA of kinetoplastids [63]. The mitochondrial DNA is packaged into an organelle, the kinetoplast, which gave the group its name as it is easily visualized, together with the nucleus, by DNA staining dyes.
Genetic Exchange in Trypanosomatids and Its Relevance to Epidemiology
2017, Genetics and Evolution of Infectious Diseases: Second EditionLiaisons dangereuses: Sexual recombination among pathogenic trypanosomes
2015, Research in MicrobiologyCitation Excerpt :Early experiments concluded that inheritance of kinetoplast DNA (kDNA) was uniparental, because analysis of the maxicircles of hybrid progeny clones showed identity to one or other of the parental genotypes [18,19,48], but subsequent analysis of the minicircle component, which consists of about 5000 intercalated 1 kb circular DNA molecules [49], showed that hybrid progeny clones had a mixture of minicircles derived from the two parents [50,51]. Therefore, contrary to initial ideas, kDNA is indeed exchanged during mating, and this was confirmed by PCR-based analysis of maxicircles of hybrid clones [37,52]. In theory, random partitioning of the small number of maxicircles relative to minicircles (estimated ratio of 50 maxicircles to 5000 minicircles per kinetoplast) would lead to uniformity of the maxicircle component after several generations without affecting the heterogeneity of the minicircles [53], but there are other explanations consistent with the experimental observations [49].
Genetic Exchange in Trypanosomatids and its relevance to Epidemiology
2011, Genetics and Evolution of Infectious DiseasesThe heart of darkness: growth and form of Trypanosoma brucei in the tsetse fly
2009, Trends in ParasitologyCitation Excerpt :Three cellular events are central to genetic exchange: (i) a meiotic cell cycle including homologous recombination; (ii) fusion of two nuclei; and (iii) reconstitution of diploidy. There is an implicit requirement for cell fusion to occur for random assortment of chromosomes and for mitochondria to fuse for genetic exchange between kinetoplasts (mitochondrial DNA) [32,46,47]. Cells undergoing meiosis and/or cell fusion have not been definitively identified, and the temporal order of meiosis and cell fusion is unknown.
Selective amplification of maxicircle classes during the life cycle of Leishmania major
2009, Molecular and Biochemical Parasitology