Regular Article
Basic Fibroblast Growth Factor Induces Cyclooxygenase-2 Expression in Endothelial Cells Derived from Bone,☆☆

https://doi.org/10.1006/bbrc.1998.9875Get rights and content

Abstract

Although histological studies have suggested that endothelial cells in bone (BDECs) are associated with some osteolytic bone diseases, it is still unclear how BDECs contribute to bone remodeling. Here we examined the response of BDECs to basic fibroblast growth factor (bFGF, FGF-2) using primary and cloned murine BDECs isolated from the femurs of BALB/c mice. Treatment of primary and cloned BDECs with bFGF induced cyclooxygenase-2 (COX-2) mRNA and protein expression. Furthermore, bFGF promotes the production of prostaglandin E2(PGE2), which is known to be a potent stimulator of bone resorption and to induce osteoclast formation. Because the secretion of PGE2was suppressed by COX-2 specific inhibitor NS-398 and by COX-2 antisense oligodeoxynucleotides, bFGF promotes the synthesis of PGE2in a COX-2-dependent manner. Therefore, endothelial cells in bone are associated with bone remodeling by controlling COX-2 expression and consequently PGE2production.

References (34)

  • G.R. Dickson et al.

    Bone

    (1990)
  • C.C. Pilbeam et al.

    J. Biol. Chem.

    (1993)
  • A. Ristimaki et al.

    J. Biol. Chem.

    (1994)
  • J.A. Angerman-Stewart et al.

    Arch. Biochem. Biophys.

    (1995)
  • S.H. Lee et al.

    J. Biol. Chem.

    (1992)
  • B.S. Fletcher et al.

    J. Biol. Chem.

    (1992)
  • E.A. Meade et al.

    J. Biol. Chem.

    (1993)
  • M. Murakami et al.

    J. Biol. Chem.

    (1997)
  • S.T. Reddy et al.

    J. Biol. Chem.

    (1994)
  • J.W. Dietrich et al.

    Prostaglandins

    (1975)
  • C. Basilico et al.

    Adv. Cancer Res.

    (1992)
  • T. Tatsuta et al.

    J. Biol. Chem.

    (1992)
  • T.J. Martin et al.

    J. Cell. Biochem.

    (1994)
  • S.C. Manolagas

    Bone

    (1995)
  • G.R. Dickson et al.

    Histochemistry

    (1987)
  • W.L. Smith et al.

    Adv. Immunol.

    (1997)
  • Cited by (57)

    • Growth factor regulation of prostaglandin-endoperoxide synthase 2 (Ptgs2) expression in colonic mesenchymal stem cells

      2010, Journal of Biological Chemistry
      Citation Excerpt :

      However, a variety of other growth factors are also likely to be capable of regulating Ptgs2 expression in this cell type. These factors include FGFs other than Fgf9 (54), epidermal growth factor (55, 56), and insulin-like growth factor (57, 58). These various growth factors may act via a number of signaling networks that have been implicated in the regulation of Ptgs2, many of which are involved in the induction of gene expression during the inflammatory response rather than in constitutive expression (59).

    • Dexamethasone inhibits basic fibroblast growth factor-stimulated gastric epithelial cell proliferation

      2008, Biochemical Pharmacology
      Citation Excerpt :

      The concentration is around 10–400 ng/ml if one estimates that 1 mg biopsy gastric specimen is about equal to the weight of 0.001 ml water. The bFGF concentrations we used in this study were 10–50 ng/ml which is also similar to other in vitro studies [14,16], and are within the physiological concentration of gastric ulcerated mucosa in humans. The concentration of dexamethasone (10−8 and 10−6 M) we used in this study was also similar to the pharmacologic concentration found in plasma of patients treated with dexamethasone [26,27].

    • Cyclooxygenase-2 and Tumor Biology

      2007, Advances in Clinical Chemistry
      Citation Excerpt :

      COX‐2 inhibition also suppresses αVβ3‐mediated Cdc42/Rac‐dependent migration and spreading of endothelial cells [49], while PGE2 promotes these activities [50]. Interestingly, VEGF and bFGF induce COX‐2 and subsequent prostaglandin production in endothelial cells [51]. Salcedo et al. showed that PGE2 mediates VEGF‐ and bFGF‐induced CXCR4‐dependent neovessel assembly in vivo [52].

    View all citing articles on Scopus

    This study was supported in part by a special grant for Advanced Research on Cancer; a Grant-in-Aid for Cancer Research from the Ministry of Education, Science, and Culture, Japan; a grant from the Ministry of Health and Welfare, Japan; and a grant from Public Trust Haraguchi Memorial Cancer Research Fund, Japan.

    ☆☆

    Abbreviations used: BDECs, endothelial cells in bone; bFGF, basic fibroblast growth factor; PG, prostaglandin; COX, cyclooxygenase; RT-PCR, reverse-transcription polymerase chain reaction; ELISA, enzyme-linked immunosorbent assay

    2

    To whom correspondence should be addressed. Fax: +81-3-3816-3592. E-mail:[email protected].

    View full text