Regular ArticleIsolation of Lamellar Aggregates of the Light-Harvesting Chlorophyll a/b Protein Complex of Photosystem II with Long-Range Chiral Order and Structural Flexibility☆
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Hierarchical organization and structural flexibility of thylakoid membranes
2014, Biochimica et Biophysica Acta - BioenergeticsCitation Excerpt :At the same time, the absorption spectrum of lamellar aggregates, if corrected for scattering and sieving, is almost superposable on the spectrum obtained after disrupting the aggregates by detergents at a concentration close to its critical micelle concentration [37].) In microcrystalline lamellar aggregates, which are largely deficient in lipids, the intensity of the new bands is usually more than an order of magnitude higher than the excitonic CD bands of the solubilized LHCII [38] (Fig. 1). The long-range order of the complexes can also be recognized by the high diamagnetic anisotropy of the samples [36], and can also be visualized using scanning transmission electron microscopy [39].
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Functional domain size in aggregates of light-harvesting complex II and thylakoid membranes
2011, Biochimica et Biophysica Acta - Bioenergetics
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P. Mathis, Ed.