Regular ArticleThe Methylation-Acetylation Method: An Ultrastructural Cytochemistry for Nucleic Acids Compatible with Immunogold Studies
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Functional alterations of root meristematic cells of Arabidopsis thaliana induced by a simulated microgravity environment
2016, Journal of Plant PhysiologyCitation Excerpt :Sample processing continued by dehydration until 100% methanol followed by the methylation-acetylation procedure, consisting in an overnight treatment at 25 °C with acetic anhydride and methanol (1:5, v/v). This technique allows a better resolution of the nucleolar ultrastructure and nucleolar subcomponents (Testillano et al., 1995). Finally, all root tips were embedded in LR White acrylic resin (London Resin Company, London, UK).
A change of developmental program induces the remodeling of the interchromatin domain during microspore embryogenesis in Brassica napus L.
2011, Journal of Plant PhysiologyCitation Excerpt :For the preferential staining of DNA, the NAMA-Ur (Testillano et al., 1991) ultrastructural cytochemistry was used. The MA method is a pre-embedding technique that highlights nucleic acid-containing structures (fibrillar and granular) by a selective blockage of the protein ability to react with uranyl acetate (Testillano et al., 1995). For this method, fixed samples were dehydrated in a methanol series and incubated in a freshly made methanol:acetic anhydride mixture (5:1 v:v) overnight at room temperature, prior to embedding.
MAPKs entry into the nucleus at specific interchromatin domains in plant differentiation and proliferation processes
2002, Journal of Structural BiologyDefined nuclear changes accompany the reprogramming of the microspore to embryogenesis
2000, Journal of Structural BiologyUltrastructural methods for nucleic acid detection by immunocytology
1999, Progress in Histochemistry and Cytochemistry