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Polymorphism of Low Mr Glutenin Subunits in Triticum tauschii

https://doi.org/10.1006/jcrs.2001.0424Get rights and content

Abstract

A collection of 173 Triticum tauschii accessions was analysed to evaluate the variability of low molecular weight (Mr) glutenin subunits. These proteins were analysed by one-step one-dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and were divided into B-, C- and D-subunits in accordance with their electrophoretic mobility. Extensive polymorphism, both in the number and electrophoretic mobility, was detected in lowMr glutenin subunits present in T. tauschii. Thirty different patterns for B-subunits and forty-three for C-subunits were identified, some of which were with identical electrophoretic mobility than those observed in hexaploid wheat. Glutenin subunits with the same electrophoretic mobilities of low Mr D-glutenin subunits as well as subunits encoded at the Glu-D4 and Glu-D5 loci, were also detected in accessions of T. tauschii. These results provide new basic knowledge regarding the genetics variability of the low Mr glutenin subunits, as well as their potential to create novel germplasm for the improvement of wheat quality in breeding programs.

References (44)

  • E.S Lagudah et al.

    Wild relatives as a source of new germplasm for cereals improvement

    In Focused plant improvement: toward responsible and sustainable agriculture. Proceedings of the 10th Australian Plant Breeding Conference

    (1993)
  • P.I Payne et al.

    Wheat storage proteins: their genetics and their potential for manipulation by plant breeding

    Philosophical Transactions of the Royal Society of London Series B Biological Sciences

    (1984)
  • P.I Payne et al.

    The relationship between HMW glutenin subunit composition and the bread-making quality of British-grown wheat varieties

    Journal of the Science of Food and Agriculture

    (1987)
  • R.B Gupta et al.

    Two-step one-dimensional SDS-PAGE analysis of LMW subunits of glutenin. I. Variation and genetic control of the subunits in hexaploid wheats

    Theoretical and Applied Genetics

    (1990)
  • S.M Masci et al.

    Two-dimensional electrophoresis of 1D-encoded B and D glutenin subunits in common wheats with similar omega gliadins

    Biochemical Genetics

    (1991)
  • E Masci et al.

    D-glutenin subunits: N-terminal sequences and evidence for the presence of cysteine

    Cereal Chemistry

    (1993)
  • G Sreeramulu et al.

    Genetic and biochemical characterization of novel low molecular weight glutenin subunits in wheat (Triticum aestivum L.)

    Genome

    (1997)
  • N.K Singh et al.

    Linkage mapping of the genes controlling endosperm proteins in wheat. 1. Genes on the short arms of group 1 chromosomes

    Theoretical and Applied Genetics

    (1988)
  • E.S Lagudah et al.

    Phylogenetic relationships of Triticum tauschii the D genome donor to hexaploid wheat 1. Variation in HMW subunits of glutenin and gliadins

    Theoretical and Applied Genetics

    (1988)
  • M.D.H.M Williams et al.

    Seed protein and isozyme variations in Triticum tauschii ( Aegilops squarrosa)

    Theoretical and Applied Genetics

    (1993)
  • M.C Gianibelli

    New Proteins for Improving Wheat Quality. Ph. D. Thesis

    Faculty of Agriculture, University of Western Sydney, Sydney, Australia.

    (1998)
  • V Neuhoff et al.

    Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie brilliant blue G-250 and R-250

    Electrophoresis

    (1988)
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    f1

    Address for correspondence: M.C. Gianibelli, CSIRO Plant Industry, PO Box 1600, ACT 2601 Australia.

    f2

    Present address: Department of Grain Science and Industry, Kansas State University, Manhattan, Kansas, KS 66506-2201, U.S.A.

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