Mucin Adsorption to Hydrophobic Surfaces

doi:10.1006/jcis.2000.6724

Journal of Colloid and Interface Science

Volume 224, Issue 2, 15 April 2000, Pages 372-381

https://doi.org/10.1006/jcis.2000.6724 Get rights and content

Abstract

The adsorption isotherm of bovine submaxillary gland mucin (BSM) onto a hydrophobic polystyrene surface was determined by using the solution depletion method, in which mucin concentrations were analyzed by amino acid analysis. Adsorption and desorption kinetics of BSM onto hydrophobic polystyrene surfaces were also studied by the solution depletion method, in which mucin solution concentrations were determined by measuring UV absorbance at a wavelength of 280 nm and by a BCA colorimetric assay with final calibration by amino acid analysis. From the adsorption isotherm, we found that the saturated surface concentration (Γ_max) was 2.3 mg/m², and the adsorption constant (K) was calculated as 0.099 (ml/mg). By using a Langmuir adsorption model and nonlinear fitting, kinetics parameters, k_on and k_off, were found to be 8.13×10⁻³ cm³ mg⁻¹ s⁻¹ and 5.67×10⁻⁴ s⁻¹, respectively. The coating was found to be very stable with very limited desorption (less than 2%) from a long-term observation (28 h). The mucin coating layer thickness was investigated by several analytical techniques: flow field–flow–fractionation, photon correlation spectroscopy, scanning electron microscopy, and atomic force microscopy. The thickness was measured as 4–5 nm, from which a monolayer coating was concluded. Finally, the weight average molecular weight of purified bovine submaxillary gland mucin (BSM) was determined as 1.6×10⁶ Da by using static light scattering.

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Citation Excerpt :
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¹: To whom correspondence should be addressed at Department of Biomedical Sciences, 105 Magruder Hall, Oregon State University, Corvallis, OR 97331.

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Regular ArticleMucin Adsorption to Hydrophobic Surfaces

Abstract

Clin. Mater.

Biomaterials

Biomaterials

J. Biol. Chem.

J. Biol. Chem.

J. Colloid Interface Sci.

Advances in Colloid and Interface Science

J. Colloid Interface Sci.

Exp. Eye Res.

Colloids and Surfaces B: Biointerfaces

Adv. Polym. Sci.

J. Biomed. Mater. Res.

Langmuir

FEMS Microbiol. Lett.

Critical Reviews in Biochemistry and Molecular Biology

Regular Article
Mucin Adsorption to Hydrophobic Surfaces