Regular ArticleA General Purification Procedure for Chemically Synthesized Oligoribonucleotides
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Distinguishing closely related amyloid precursors using an RNA aptamer
2014, Journal of Biological ChemistryCitation Excerpt :Methanol was removed under vacuum and the RNA pellet re-suspended in anhydrous dimethyl sulfoxide. One volume of triethylamine trihydrofluoride was added and incubated at room temperature to remove t-butyldimethylsilyl, the deprotected RNA was precipitated with butan-1-ol and resuspended in diethylpyrocarbonate-treated water (Severn Biotech) before being purified by reverse-phase HPLC at 55 °C (34). RNA fractions were collected, lyophilized, and desalted into 18.2 megohm H2O.
Structure determination and dynamics of protein-RNA complexes by NMR spectroscopy
2011, Progress in Nuclear Magnetic Resonance SpectroscopyCitation Excerpt :Certain biologically relevant RNAs might fold into different conformations or might form multimers, which can be separated by purifying them under non-denaturing conditions using size-exclusion FPLC [123,124,127]. In addition to reverse-phase HPLC [128], the use of affinity chromatography has been described [129–132]. Batey and Kieft developed a sophisticated approach, where an affinity tag is attached to the 3′-end of the RNA by a glucosamine-6-phosphate activated (glmS) ribozyme [130].
Viral Genomic Single-Stranded RNA Directs the Pathway Toward a T = 3 Capsid
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2008, Journal of Molecular BiologyThe Three-dimensional Structure of Genomic RNA in Bacteriophage MS2: Implications for Assembly
2008, Journal of Molecular Biology