Plant Soil Environ., 2005, 51(5):220-225 | DOI: 10.17221/3577-PSE

Aluminium induced esterase activity and isozyme pattern in barley root tip

L. Tamás, J. Huttová, I. Mistrík, M. Šimonovičová, B. Široká
Institute of Botany, Slovak Academy of Sciences, Bratislava, Slovakia

Changes in the activity of esterase as well as changes in the viability of root cells and some growth parameters were analysed during cultivation of barley seedlings in the artificial substrate under Al stress conditions. Aluminium-induced elevated esterase activity correlated with Al uptake, root growth inhibition and increased Evans blue uptake in the barley root tips. Analysis of isozyme pattern of esterase revealed one anodic and one cathodic esterase isozyme induced by Al-treatment. The possible role of elevated esterase activity during Al stress is discussed.

Keywords: aluminium uptake; cell death; esterase activity; isoesterases

Published: May 31, 2005  Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Tamás L, Huttová J, Mistrík I, Šimonovičová M, Široká B. Aluminium induced esterase activity and isozyme pattern in barley root tip. Plant Soil Environ.. 2005;51(5):220-225. doi: 10.17221/3577-PSE.
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Aldridge W.N. (1993): The esterases: perspectives and Al problems. Chemical and Biological Interactions, 87: 5-13. Baker C.J., Mock N.M. (1994): An improved method for monitoring cell death in cell suspension and leaf disc assays using Evans blue. Plant Cell, Tissue and Organ Culture, 39: 7-12. Go to original source...
    2. Bordenave M., Goldberg R., Heut J.C., Pernollet J.C. (1995): A novel protein from mung bean hypocotyl cell walls with acetyl esterase activity. Phytochemistry, 38: 315-319. Go to original source... Go to PubMed...
    3. Bradford M. (1976): A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72: 248-254. Go to original source...
    4. Chibbar R.N., Shyluk J., Georges F., Mallard C.S., Constabel F. (1988): Esterase isozymes as markers of somatic embryogenesis in cultured carrot cells. Journal of Plant Physiology, 133: 367-370. Go to original source...
    5. Coppens L., Dewitte D. (1990): Esterase and peroxidase zymograms from barley (Hordeum vulgare L.) callus as a biochemical marker system of embryogenesis and organogenesis. Plant Science, 67: 97-105. Go to original source...
    6. Cosgrove D.J. (2001): Wall structure and wall loosening. A look backwards and forwards. Plant Physiology, 125: 131-134. Go to original source... Go to PubMed...
    7. Cummins I., Burnet M., Edwards R. (2001): Biochemical characterisation of esterases active in hydrolysing xenobiotics in wheat and competing weeds. Physiologia Plantarum, 113: 477-485. Go to original source...
    8. Delise G., Champoux M., Houde M. (2001): Characterization of oxalate oxidase and cell death in Al-sensitive and tolerant wheat roots. Plant and Cell Physiology, 42: 324-333. Go to original source... Go to PubMed...
    9. García-Conesa M.T., Ostergaard P., Kauppinen S., Williamson G. (2001): Hydrolysis of diethyl diferulates by a tannase from Aspergillus oryzae. Carbohydrate Polymers, 44: 319-324. Go to original source...
    10. Horst W.J. (1995): The role of apoplast in aluminium toxicity and resistance of higher plants: a review. Zeitschrift für Pflanzenernährung und Bodenkunde, 158: 419-428. Go to original source...
    11. Hwang B.K., Wolf G., Heitefuss R. (1982): Soluble proteins and multiple forms of esterases in leaf tissue at first and flag stages of spring barley plants in relation to their resistance to powdery mildew (Erysiphe graminis f.sp. hordei). Physiological Plant Pathology, 21: 367-372. Go to original source...
    12. Kim J., Carpita N.C. (1992): Changes in esterification of the uronic acid groups of cell wall polysaccharides during elongation of maize coleoptiles. Plant Physiology, 98: 646-653. Go to original source... Go to PubMed...
    13. Laemmli U.K. (1970): Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature, 277: 680-685. Go to original source... Go to PubMed...
    14. Marienfeld S., Stelzer R. (1993): X-ray microanalyses in roots of Al-treated Avena sativa plants. Journal of Plant Physiology, 141: 569-573. Go to original source...
    15. Matsumoto H. (2000): Cell biology of aluminum toxicity and tolerance in higher plants. International Review of Cytology, 200: 1-46. Go to original source... Go to PubMed...
    16. Micheli F. (2001): Pectin methylesterases: cell wall enzymes with important roles in plant physiology. Trends in Plant Sciences, 6: 414-419. Go to original source... Go to PubMed...
    17. Millard M.M., Foy C.D., Coradetti C.A., Reinsel M.D. (1993): X/ray photoelectron spectroscopy surface analysis of aluminium ion stress in barley roots. Plant Physiology, 93: 578-583. Go to original source... Go to PubMed...
    18. Mukherjee S., Bhattacharyya P., Duttagupta A.K. (2004): Heavy metal levels and esterase variations between metal-exposed and unexposed duckweed Lemna minor: field and laboratory studies. Environmental Interactions, 30: 811-814. Go to original source... Go to PubMed...
    19. Nari J., Noat G., Ricard J. (1991): Pectin methylesterase, metal ions and plant cell-wall extension. Hydrolysis of pectin by plant cell-wall pectin methylesterase. Biochemical Journal, 279: 343-350. Go to original source... Go to PubMed...
    20. Ownby J.D. (1993): Mechanism of reaction of hematoxylin with aluminium-treated wheat roots. Physiologia Plantarum, 87: 371-380. Go to original source...
    21. Pringle D., Dickstein R. (2004): Purification of ENOD8 proteins from Medicago sativa root nodules and their characterization as esterase. Plant Physiology and Biochemistry, 42: 73-79. Go to original source... Go to PubMed...
    22. Reisfeld R.A., Levis U.J., Williams D.E. (1962): Disk electrophoresis of basic proteins and peptides on polyacrylamide gels. Nature, 195: 281-283. Go to original source... Go to PubMed...
    23. Rout G.R., Samantaray S., Das P. (2001): Aluminum toxicity in plants: a review. Agronomie, 21: 3-21. Go to original source...
    24. Schmohl N., Pilling J., Fisahn J., Horst W.J. (2000): Pectin methylesterase modulates aluminium sensitivity in Zea mays and Solanum tuberosum. Physiologia Plantarum, 109: 419-427. Go to original source...
    25. Silva I.R., Smyth T.J., Moxley D.F., Carter T.E., Allen N.S., Rufty T.W. (2000): Aluminum accumulation at nuclei of cells in root tip. Fluorescence detection usinglumogallion and confocal laser scanning microscopy. Plant Physiology, 124: 543-552. Go to original source... Go to PubMed...
    26. Ward R.E., Bamforth C.W. (2002): Esterases in barley and malt. Cereal Chemistry, 79: 681-686. Go to original source...

    This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY NC 4.0), which permits non-comercial use, distribution, and reproduction in any medium, provided the original publication is properly cited. No use, distribution or reproduction is permitted which does not comply with these terms.


    Return to the content