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Role of hydrogen peroxide and ascorbate-glutathione pathway in host resistance to bacterial wilt of eggplant

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Abstract

Ralstonia solanacearum, a soil-borne bacterium causes bacterial wilt, is a lethal disease of eggplant (Solanum melongena L.). However, the first line of defense mechanism of R. solanacearum infection remains unclear. The present study focused on the role of induced H2O2, defense-related enzymes of ascorbate-glutathione pathway variations in resistant and susceptible cultivars of eggplant under biotic stress. Fifteen cultivars of eggplant were screened for bacterial wilt resistance, and the concentration of antioxidant enzymes were estimated upon infection with R. solanacearum. A quantitative real-time PCR was also carried out to study the expression of defense genes. The concentration of H2O2 in the pathogen inoculated seedlings was two folds higher at 12 h after pathogen inoculation compared to control. Antioxidant enzymes of ascorbate-glutathione pathway were rapidly increased in resistant cultivars followed by susceptible and highly susceptible cultivars upon pathogen inoculation. The enzyme activity of ascorbate-glutathione pathway correlates by amplification of their defense genes along with pathogenesis-related protein-1a (PR-1a). The expressions of defense genes increased 2.5−3.5 folds in resistant eggplant cultivars after pathogen inoculation. The biochemical and molecular markers provided an insight to understand the first line of defense responses in eggplant cultivars upon inoculation with the pathogen.

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Abbreviations

ANOVA:

analysis of variance

APX:

ascorbate peroxidase

BYMV:

Bean yellow mosaic virus

DHAR:

dehydroascorbate reductase

GR:

glutathione reductase

H2O2 :

hydrogen peroxide

HS:

highly susceptible

MDHAR:

monodehydroascorbate reductase

PR:

pathogen-related

R:

resistance

ROS:

reactive oxygen species

S:

susceptible

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Authors and Affiliations

  1. Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysore, 570006, Karnataka, India

    P. Avinash, S. Umesha & S. Marahel

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  1. P. Avinash
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  2. S. Umesha
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  3. S. Marahel
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Correspondence to S. Umesha.

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Avinash, P., Umesha, S. & Marahel, S. Role of hydrogen peroxide and ascorbate-glutathione pathway in host resistance to bacterial wilt of eggplant. Russ J Plant Physiol 64, 375–385 (2017). https://doi.org/10.1134/S1021443717030049

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