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Increased Productivity and Antifreeze Activity of Ice-binding Protein from Flavobacterium frigoris PS1 Produced using Escherichia coli as Bioreactor

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Abstract

Ice-binding proteins (IBPs) inhibit the growth and recrystallization of intracellular ice, enabling polar organisms to survive at subzero temperatures. IBPs are promising materials in biomedical applications such as cryopreservation and the hypothermic storage of cells, tissues, and organs. In this study, recombinant IBP from the antarctic bacterium Flavobacterium frigoris PS1 (FfIBP) was produced by Escherichia coli used as bioreactor, to examine the feasibility of scale-up. Oxygen transfer was the most important factor influencing cell growth and FfIBP production during pilot-scale fermentation. The final yield of recombinant FfIBP produced by E. coli harboring the pET28a-FfIBP vector system was 1.6 g/L, 3.8-fold higher than that from the previously published report using pCold I-FfIBP vector system, and its thermal hysteresis activity was 2.5°C at 9.7 µM. This study demonstrates the successful pilot-scale production of FfIBP.

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ACKNOWLEDGMENTS

We thank Prof. E.S. Jin (Hanyang University) for her generous support and use of facilities, and Mr. M.J. Kim (Hanyang University) for his technical assistance in the use of the osmometer. This research was supported by research projects (PE18180 and PE18210) from the Korea Polar Research Institute, Incheon, Korea.

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Authors and Affiliations

  1. Division of Polar Life Sciences, Korea Polar Research Institute, KIOST, 21990, Incheon, South Korea

    E. J. Kim, J. E. Kim, I.-C. Kim, S. Kim & S. J. Han

  2. Department of Polar Sciences, University of Science and Technology, 21990, Incheon, South Korea

    E. J. Kim, J. S. Hwang, S. G. Lee, J. H. Lee & S. J. Han

  3. Department of Pharmacy, Graduate School, Sungkyunkwan University, 16419, Suwon, South Korea

    J. E. Kim

  4. Unit of Polar Genomics, Korea, Polar Research Institute, 21990, Incheon, South Korea

    J. S. Hwang, S. G. Lee & J. H. Lee

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  1. E. J. Kim
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  4. I.-C. Kim
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Correspondence to S. J. Han.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Kim, E.J., Kim, J.E., Hwang, J.S. et al. Increased Productivity and Antifreeze Activity of Ice-binding Protein from Flavobacterium frigoris PS1 Produced using Escherichia coli as Bioreactor. Appl Biochem Microbiol 55, 489–494 (2019). https://doi.org/10.1134/S0003683819050077

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