1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 59, Issue 4

gen. nov., sp. nov., a novel anamorphic fungus containing an S943 nuclear small-subunit rRNA group IB intron represents a basal branch of Microstromatales Free

Abstract

In the course of molecular characterization of yeasts isolated from decaying leaves collected in Cambodia, anamorphic yeast strains were detected that clustered with the Microstromatales in molecular phylogenetic analyses of the chromosomal regions coding for the D1/D2 domain of the large-subunit 26S rRNA gene, the small-subunit 18S rRNA gene and the internal transcribed spacer ITS1-5.8S rRNA gene-ITS2. In the trees obtained, the isolates formed a distinct, basal branch of Microstromatales supported with high bootstrap values. As the isolates could not be identified with any known genus, we have described them as gen. sp. nov. (type strain C5b=CBS 10918=CCY 88-1-1). In the chromosomal region coding for the 18S rRNA gene, the type strain of the novel species contained a group IB intron that was similar in location and sequence to introns found in certain species of Exobasidiales and Entylomatales. As no similar introns have been detected in Microstromatales, the new genus might represent a phylogenetic link connecting these three orders.

  • Published Online:
Keyword(s): ITS, internal transcribed spacer
© SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.003939-0
2009-04-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/4/914.html?itemId=/content/journal/ijsem/10.1099/ijs.0.003939-0&mimeType=html&fmt=ahah

References

  1. Bauer, R., Oberwinkler, F. & Vanky, K.(1997). Ultrastructural markers and systematics in smut fungi and allied taxa. Can J Bot 75, 1273–1314.[CrossRef] [Google Scholar]
  2. Begerow, D., Bauer, R. & Oberwinkler, F.(2001). Muribasidiospora: microstromatales or exobasidiales? Mycol Res 105, 798–810.[CrossRef] [Google Scholar]
  3. Begerow, D., John, B. & Oberwinkler, F.(2004). Evolutionary relationships among β-tubulin gene sequences of basidiomycetous fungi. Mycol Res 108, 1257–1263.[CrossRef] [Google Scholar]
  4. Begerow, D., Stoll, M. & Bauer, R.(2006). A phylogenetic hypothesis of Ustilaginomycotina based on multiple gene analyses and morphological data. Mycologia 98, 906–916.[CrossRef] [Google Scholar]
  5. Bhattacharya, D., Reeb, V., Simon, D. M. & Lutzoni, F.(2005). Phylogenetic analyses suggests reverse splicing spread of group I introns in fungal ribosomal DNA. BMC Evol Biol 5, 68[CrossRef] [Google Scholar]
  6. Boekhout, T., Theelen, B., Houbraken, J., Robert, V., Scorzetti, G., Gafni, A., Gerson, U. & Sztejnberg, A.(2003). Novel anamorphic mite-associated fungi belonging to the Ustilaginomycetes: Meira geulakonigii gen. nov., sp. nov., Meira argovae sp. nov. and Acaromyces ingoldii gen. nov., sp. nov. Int J Syst Evol Microbiol 53, 1655–1664.[CrossRef] [Google Scholar]
  7. Boekhout, T., Gildemacher, P., Theelen, B., Muller, W. H., Heijne, B. & Lutz, M.(2006). Extensive colonization of apples by smut anamorphs causes a new post-harvest disorder. FEMS Yeast Res 6, 63–76.[CrossRef] [Google Scholar]
  8. Cech, T. R.(1988). Conserved sequences and structures of group I introns: building an active site for RNA catalysis – a review. Gene 73, 259–271.[CrossRef] [Google Scholar]
  9. De Wachter, R., Neefs, J.-M., Goris, A. & Van der Peer, Y.(1992). The gene coding for small ribosomal subunit RNA in the basidiomycete Ustilago maydis contains a group I intron. Nucleic Acids Res 20, 1251–1257.[CrossRef] [Google Scholar]
  10. Fell, J. W., Boekhout, T., Fonseca, A., Scorzetti, G. & Statzell-Tallman, A.(2000). Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis. Int J Syst Evol Microbiol 50, 1351–1371.[CrossRef] [Google Scholar]
  11. Felsenstein, J.(2007).phylip (phylogeny inference package), version 3.67. Department of Genome Sciences, University of Washington, Seattle, USA.
  12. Haugen, P., Reeb, V., Lutzono, F. & Bhattacharya, D.(2004). The evolution of homing endonuclease genes and group I introns in nuclear rDNA. Mol Biol Evol 21, 129–140. [Google Scholar]
  13. Katoh, K. & Toh, H.(2008). Recent developments in the MAFFT multiple sequence alignment program. Brief Bioinform 9, 286–298.[CrossRef] [Google Scholar]
  14. Kulakovskaya, T. V., Shashkov, A. S., Kulakovskaya, E. V. & Golubev, W. I.(2004). Characterization of an antifungal glycolipid secreted by the yeast Sympodiomycopsis paphiopedili. FEMS Yeast Res 5, 247–252.[CrossRef] [Google Scholar]
  15. Kurtzman, C. P. & Robnett, C. J.(1998). Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 73, 331–371.[CrossRef] [Google Scholar]
  16. Matheny, P. B., Wang, Z., Binder, M., Curtis, J. M., Lim, Y. W., Nilsson, R. H., Hughes, K. W., Hofstetter, V., Ammirati, J. F. & other authors(2007). Contributions of rpb2 and tef1 to the phylogeny of mushrooms and allies (Basidiomycota, Fungi). Mol Phylogenet Evol 43, 430–451.[CrossRef] [Google Scholar]
  17. O'Donnell, K.(1993).Fusarium and its near relatives. In The Fungal Holomorph: Mitotic, Meiotic and Pleomorphic Speciation in Fungal Sytematics, pp. 225–233. Edited by D. R. Reynolds & J. W. Taylor. Wallingford, UK: CAB International.
  18. Page, R. D. M.(1996). TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12, 357–358. [Google Scholar]
  19. Scorzetti, G., Fell, J. W., Fonseca, A. & Statzell-Tallman, A.(2002). Systematics of basidiomycetous yeasts: a comparison of large subunit D1/D2 and internal transcribed spacer rDNA regions. FEMS Yeast Res 2, 495–517.[CrossRef] [Google Scholar]
  20. Sipiczki, M.(2003).Candida zemplinina sp. nov., an osmotolerant and psychrotolerant yeast that ferments sweet botrytized wines. Int J Syst Evol Microbiol 53, 2079–2083.[CrossRef] [Google Scholar]
  21. Sipiczki, M.(2006).Metschnikowia strains isolated from botrytized grapes antagonize fungal and bacterial growth by iron depletion. Appl Environ Microbiol 72, 6716–6724.[CrossRef] [Google Scholar]
  22. Sipiczki, M., Takeo, K., Yamaguchi, M., Yoshida, S. & Miklos, I.(1998). Environmentally controlled dimorphic cycle in a fission yeast. Microbiology 144, 1319–1330.[CrossRef] [Google Scholar]
  23. Spadaro, D. & Gullino, M. L.(2004). State of the art and future prospects of the biological control of postharvest fruit disease. Int J Food Microbiol 91, 185–194.[CrossRef] [Google Scholar]
  24. Sugiyama, J., Tokuoka, K., Suh, S.-O., Hirata, A. & Komagata, K.(1991).Sympodiomycopsis: a new yeast-like anamorph genus with basidiomycetous nature from orchid nectar. Antonie van Leeuwenhoek 59, 95–108.[CrossRef] [Google Scholar]
  25. Suh, S.-O. & Sugiyama, J.(1994). Phylogenetic placement of the basidiomycetous yeasts Kondoa malvinella and Rhodosporidium dacryoidum, and the anamorph yeast Sympodiomycopsis paphiopedili by means of 18S rRNA gene sequence analysis. Mycoscience 35, 367–375.[CrossRef] [Google Scholar]
  26. Thompson, J. D., Higgins, D. G. & Gibson, T. J.(1994).clustalw: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef] [Google Scholar]
  27. van der Walt, J. P. & Yarrow, D.(1984). Methods for the isolation, maintenance, classification and identification of yeasts. In The Yeasts: a Taxonomic Study, pp. 45–104. Edited by N. J. W. Kreger-van Rij. Amsterdam: Elsevier.
  28. Weijman, A. C., Rodrigues de Miranda, L. & van der Walt, J. P.(1988). Redefinition of Candida Berkhout and the consequent emendation of Cryptococcus Kutzing and Rhodotorula Harrison. Antonie van Leeuwenhoek 54, 545–553.[CrossRef] [Google Scholar]
  29. White, T. J., Burns, T., Lee, S. & Taylor, J.(1990). Amplification and sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols. A Guide to Methods and Applications, pp. 315–322. Edited by M. A. Innis, D. H. Gelfand, J. J. Snisky & T. J. White. San Diego, CA: Academic Press.
  30. Xu, J., Saunders, C. W., Hu, P., Grant, R. A., Boekhout, T., Kuramae, E. E., Kronstad, J. W., Deangelis, Y. M., Reeder, N. L. & other authors(2007). Dandruff-associated Malassezia genomes reveal convergent and divergent virulence traits shared with plant and human fungal pathogens. Proc Natl Acad Sci U S A 104, 18730–18735.[CrossRef] [Google Scholar]
  31. Zimmer, C., Platz, T., Cadez, N., Giffhorn, F. & Kohring, G. W.(2006). A cold active (2R,3R)-(−)-di-O-benzoyl-tartrate hydrolyzing esterase from Rhodotorula mucilaginosa. Appl Microbiol Biotechnol 73, 132–140.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.003939-0
Loading
Jaminaea angkorensis gen. nov., sp. nov., a novel anamorphic fungus containing an S943 nuclear small-subunit rRNA group IB intron represents a basal branch of Microstromatales
Int J Syst Evol Microbiol 59, 914 (2009); https://doi.org/10.1099/ijs.0.003939-0
/content/journal/ijsem/10.1099/ijs.0.003939-0
/content/journal/ijsem/10.1099/ijs.0.003939-0
Loading

Data & Media loading...

Supplements

CLUSTAL alignment of the rRNA group 1 introns of and gen. nov., sp. nov., strain C5b . [PDF](44 KB)

PDF

Phylogenetic analysis of S943 nuclear small-subunit rRNA group IB intron sequences using the maximum-likelihood method. [PDF](10 KB)

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error