Molecular & Cellular Proteomics
ResearchN-glycosylation Profiling of Colorectal Cancer Cell Lines Reveals Association of Fucosylation with Differentiation and Caudal Type Homebox 1 (CDX1)/Villin mRNA Expression*
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Author contributions: S.H., A.M.D., Y.R., and M.W. the designed research; S.H., A.J.D., G.W.v., S.J.v., and J.J.G. performed the research; C.A.K., W.E.M., and R.A.T. contributed new reagents or analytic tools; S.H., J.J.G., and C.A.K. analyzed the data; S.H., G.W.v., Y.R., and M.W. wrote the paper; and A.J.D. performed method optimization.
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This work was supported by the European Union (Seventh Framework Programme HighGlycan project, grant number: 278535).
This article contains supplemental material Supplemental Tables S1-S8 and Supplemental Figs. S1-S7.
- 1
The abbreviations used are:
- CRC
colorectal cancer
- DHB
2,5-dihydroxybenzoic acid
- dHex
deoxyhexose
- DMEM
Dulbecco's Modified Eagle
- F
Fuc, fucose
- FCS
fetal calf serum
- GalNAc
N-acetylgalactosamine
- GlcNAc
N-acetylglucosamine
- GuHCl
guanidine hydrochloride
- H
Hex, hexose
- N
HexNAc, N-acetylhexosamine
- HILIC
hydrophilic interaction liquid chromatography
- LC-ESI-MS
liquid chromatography-electrospray ionization-mass spectrometry
- MALDI-TOF-MS
matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
- MS/MS
tandem mass spectrometry
- NeuAc
N-acetylneuraminic acid
- PCA
principal component analysis
- PNGase F
peptide N-glycosidase F
- RPMI
Roswell Park Memorial Institute
- SPE
solid phase extraction.