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Analysing differential gene expression in cancer

Nature Reviews Cancer volume 3, pages 869–876 (2003)Cite this article

Abstract

Analysis of messenger RNA and proteins is widely used to compare patterns of gene expression between cells or tissues of different kinds and under different conditions; for example, between normal and cancer cells. The goal of the individuals who are developing these methods has been to enable faster, simpler, more sensitive and systematic analyses, and over the past few decades techniques have become increasingly more sophisticated. This timeline article reviews the evolution of these technologies as well as strategies for identifying differentially expressed genes in normal and cancer cells. It also highlights their use for the search for target genes of the tumour suppressor p53.

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Figure 1: Differential hybridization.
Figure 2: Subtractive hybridization.
Figure 3: Differential display.
Figure 4: DNA micrarrays.
Figure 5: SAGE.

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Author information

Authors and Affiliations

  1. the Department of Cancer Biology, Vanderbilt–Ingram Cancer Center, School of Medicine, Vanderbilt University, Nashville, 37232, Tennessee, USA

    Peng Liang

  2. the Department of Adult Oncology, Dana–Farber Cancer Institute, 44 Binney Street, Boston, 02115, Massachusetts, USA

    Arthur B. Pardee

Authors
  1. Peng Liang
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  2. Arthur B. Pardee
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Related links

Related links

DATABASES

Cancer.gov

breast cancer

leukaemia

lung cancer

lymphoma

prostate cancer

LocusLink

BAX

ERBB

GADD45

IL-24

MDM2

p53

p68

PIRH2

RAS

v-REL

WAF1

FURTHER INFORMATION

Beads-based EST sequencing

cDNA microarray

Differential display

GeneChip array

International Symposia on Differential Gene Expression

SAGE

SAGE Genie

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Liang, P., Pardee, A. Analysing differential gene expression in cancer. Nat Rev Cancer 3, 869–876 (2003). https://doi.org/10.1038/nrc1214

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