Abstract
Neutral oligosaccharides in human milk samples from approximately 50 women were analysed applying a recently developed high-pH anion-exchange chromatographic method. Three different oligosaccharide patterns could be detected in accordance with milk groups that had been already described. These oligosaccharide groups correspond to the Lewis blood types Le(a−b+), Le(a+b−) and Le(a−b−). In addition to these oligosaccharide patterns, a new carbohydrate pattern was detected in a milk sample from a Le(a−b−) individual. Here, only nonfucosylated oligosaccharides and compounds bearing a1,3 linked fucosyl residues were found, whereas structures with a1,2 and a1,4 fucosyl linkages were missing. This finding led to the hypothesis that there are four different oligosaccharide milk groups that fit well to the genetic basis of the Lewis blood group system.
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Stahl B, Thurl S, Zeng J, Karas M, Hillenkamp F, Steup M, Sawatzki G (1994) Anal Biochem 223: 218-26.
Kobata A (1973) Methods Enzymol 28: 262-71.
Bruntz R, Dabrowski U, Dabrowski J, Ebersold A, Peter-Katalinic J, Egge H (1988) Biol Chem Hoppe-Seyler 369: 257-73.
Haeuw-Fievre S, Wieruszeski JM, Plancke Y, Michalski JC, Montreuil J, Strecker G (1993) Eur J Biochem 215: 361-71.
Kunz C, Rudloff S (1993) Acta Paediatr 82: 903-12.
Kobata A (1992) Eur J Biochem 209: 483-501.
Oriol R, Le Pendu J, Mollicone R (1986) Vox Sang 51: 161-71.
Johnson PH, Watkins WM (1992) Glycoconj J 9: 241-9.
Henry SM, Oriol R, Samuelsson BE (1994) Glycoconj J 11: 593-9.
Lee YC (1990) Anal Biochem 189: 151-62.
Wang WT, Zopf D (1989) Carbohydr Res 189: 1-11.
Reddy GP, Bush CA (1991) Anal Biochem 198: 278-84.
Thurl S, Müller-Werner B, Sawatzi G (1996) Anal Biochem 235: 202-6.
Thurl S, Offermanns J, Müller-Werner B, Sawatzki G (1991) J Chromatogr 568: 291-300
Viverge D, Grimmonprez L, Cassanas G, Bardet L, Solere M (1990) J Pediatr Gastroenterol Nutr 11: 365-70.
Eppenberger-Castori S, Lötscher H, Finne J (1989) Glycoconj J 6: 101-14.
Mollicone R, Gibaud A, Francois A, Ratcliffe M, Oriol R (1990) Eur J Biochem 191: 169-76.
Mollison PL (1979) Blood Transfusion in Clinical Medicine, 6th Ed. Oxford: Blackwell.
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Thurl, S., Henker, J., Siegel, M. et al. Detection of four human milk groups with respect to Lewis blood group dependent oligosaccharides. Glycoconj J 14, 795–799 (1997). https://doi.org/10.1023/A:1018529703106
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DOI: https://doi.org/10.1023/A:1018529703106