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Treatment with roscovitine and butyrolactone I prior to in vitro maturation alters blastocyst production

Published online by Cambridge University Press:  11 October 2019

Rosiara Rosária Dias Maziero Open the ORCID record for Rosiara Rosária Dias Maziero [Opens in a new window] ,
Carlos Renato de Freitas Guaitolini ,
Daniela Martins Paschoal ,
André Maciel Crespilho ,
Bianca Andriolo Monteiro ,
Jonathan Soares de Lima ,
Danielle Andressa Oliveira Sestari  and
Fernanda da Cruz Landim-Alvarenga
Rosiara Rosária Dias Maziero*
Affiliation:
Paranaense University, UNIPAR, Umuarama, Paraná, Brazil São Paulo State University, UNESP, School of Veterinary Medicine and Animal Science, FMVZ, Department of Animal Reproduction and Veterinary Radiology, Botucatu, São Paulo, Brazil
Carlos Renato de Freitas Guaitolini
Affiliation:
Paranaense University, UNIPAR, Umuarama, Paraná, Brazil São Paulo State University, UNESP, School of Veterinary Medicine and Animal Science, FMVZ, Department of Animal Reproduction and Veterinary Radiology, Botucatu, São Paulo, Brazil
Daniela Martins Paschoal
Affiliation:
São Paulo State University, UNESP, School of Veterinary Medicine and Animal Science, FMVZ, Department of Animal Reproduction and Veterinary Radiology, Botucatu, São Paulo, Brazil
André Maciel Crespilho
Affiliation:
Santo Amaro University, UNISA, São Paulo, São Paulo, Brazil
Bianca Andriolo Monteiro
Affiliation:
São Paulo State University, UNESP, School of Veterinary Medicine and Animal Science, FMVZ, Department of Animal Reproduction and Veterinary Radiology, Botucatu, São Paulo, Brazil
Jonathan Soares de Lima
Affiliation:
Paranaense University, UNIPAR, Umuarama, Paraná, Brazil
Danielle Andressa Oliveira Sestari
Affiliation:
Paranaense University, UNIPAR, Umuarama, Paraná, Brazil
Fernanda da Cruz Landim-Alvarenga
Affiliation:
São Paulo State University, UNESP, School of Veterinary Medicine and Animal Science, FMVZ, Department of Animal Reproduction and Veterinary Radiology, Botucatu, São Paulo, Brazil
*
Address for correspondence: Rosiara Rosária Dias Maziero. Paranaense University, Umuarama, Paraná, Brazil. Rua Leonildo Stecca, 2589, Jardim Cruzeiro, Cep: 87504-580. Tel: +55 44991681568. E-mail: rosiaramaziero@prof.unipar.br
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Summary

This study evaluated the effects of oocyte meiosis inhibitors roscovitine (ROS) and butyrolactone I (BL-I) on in vitro production of bovine embryos. Bovine oocytes were maintained in pre in vitro maturation (pre-IVM) with 25 µM ROS or 100 µM BL-I for 24 h to delay meiosis and for 24 h in in vitro maturation (IVM). Following this treatment, the nuclear maturation index was evaluated. All embryos degenerated following this procedure. In the second set of experiments, oocytes were maintained for 6 or 12 h in pre-IVM with the following three treatments: ROS (25 µM or 12.5 µM), BL-I (100 µM or 50 µM) or a combination of both drugs (6.25 µM ROS and 12.5 µM BL-I). Oocytes were cultivated for 18 or 12 h in IVM. When a meiosis-inducing agent was used during pre-IVM for 24 h, more degenerated oocytes were observed at the end of the IVM period. This effect decreased when the meiotic blocking period was reduced to 6 or 12 h. No significant differences were observed in the blastocyst production rate of oocytes in pre-IVM for 6 h with ROS, BL-I, or ROS + BL-I compared with that of the control group (P > 0.05). However, inhibition of oocytes for 12 h resulted in decreased embryo production compared with that in the controls (P < 0.05). There was no difference in the post-vitrification embryo re-expansion rate between the study groups, showing that the meiotic inhibition for 6 or 12 h did not alter the embryo cryopreservation process.

Keywords

BovineEmbryo productionMeiotic inhibitorsNuclear maturationOocyteVitrification
Type
Research Article
Information
Zygote , Volume 28 , Issue 1 , February 2020 , pp. 24 - 31
Copyright
© Cambridge University Press 2019 

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