Basic ResearchNew Bacterial Composition in Primary and Persistent/Secondary Endodontic Infections with Respect to Clinical and Radiographic Findings
Section snippets
Patient Selection
Twenty-one patients (25–75 years of age) who had been referred to the University of Freiburg–Medical Center, Freiburg, Germany, for endodontic treatment took part in this study. Patients were excluded from the study if they showed 1 of the following criteria: severe systemic diseases, pregnancy or lactation, use of any antibiotics within the past 30 days, participation in another clinical study during the previous 3 months, teeth that could not be isolated with a rubber dam, teeth without a
Results
In 12 of 21 root canals, 33 different bacterial species could be isolated (Table 2). Six (50%) of the cases with isolated microorganisms were associated with primary endodontic infections, and 6 (50%) with secondary/persistent endodontic infections. The CFUs ranged from 1 × 103 to 1.49 × 107 CFU/mL. The largest number of microorganisms was isolated from a tooth with a chronic apical abscess. In 2 of 6 cases with an intracanal dressing, either Ledermix or calcium hydroxide, microorganisms were
Discussion
Although there are many reports addressing the microbiota in endodontic infections up to now, only few studies described the bacterial prevalence in German patients 12, 13, 14, 15. Moreover, only few studies on the endodontic infections correlated with chronic apical abscesses have been published 15, 16, 17, 18. In the present clinical in vivo study, microorganisms were isolated from teeth with a primary or secondary/persistent endodontic infections, and the isolated microorganisms were
Acknowledgments
The authors thank Dr Annette Anderson for her assistance in reviewing the manuscript.
Supported by the German Research Foundation (DFG, AL 1179/1-1).
The authors deny any conflicts of interest related to this study.
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