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Genome-wide characterization and expression profiling of TIFY gene family in pigeonpea (Cajanus cajan (L.) Millsp.) under copper stress

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Abstract

Copper is a vital micronutrient for plant growth and development, but in high concentration it leads to the oxidative damage thereby initiating biosynthesis of jasmonic acid (JA). The JA signalling is mediated by various transcription factors (TFs) such as JAZ and MYC2. JAZ TFs are the negative regulators of JA signalling which are degraded in presence of JA. JAZ proteins belong to TIFY family and regulate various biological processes such as plant development, response to phytohormones, biotic and abiotic stresses. In the present study, we identified 18 TIFY family proteins in pigeonpea, which are further classified into TIFY, JAZ and ZML subfamilies. Gene expression of 12 CcTIFY genes in pigeonpea was studied under methyl jasmonate (Me-JA), JA in presence and absence of copper (Cu). Our results showed that the transcript levels of CcTIFY3, CcTIFY4, CcTIFY5, CcTIFY9 and CcTIFY16 were upregulated under Cu stress, indicating their involvement in Cu stress signalling. The presence of defense and stress responsive cis-regulatory elements in the promoter regions of these genes further confirms their involvement in response to Cu stress. Apart from feedback regulation of JAZ proteins, the expression of CcTIFY3 and CcTIFY9 was observed to be upregulated in Me-JA and JA treatment, respectively, which suggest involvement of alternative JA and Me-JA signalling.

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Abbreviations

JA:

Jasmonic Acid

Me-JA:

Methyl jasmonate

qRT-PCR:

Quantitative real-time polymerase chain reaction

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Acknowledgments

GS acknowledges financial support from the Science and Engineering Research Board, Department of Science and Technology (DST), Government of India. Research in the laboratory of AKS and VA is supported by the grants of Council of Scientific and Industrial Research (CSIR). PS thanks the DST for financial support in the form of WOS-A project. PG thanks CSIR for providing fellowship. PA and GK thank University Grants Commission for providing fellowship. This manuscript represents CSIR-IHBT communication number 3854.

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Authors and Affiliations

  1. Department of Botany, Punjabi University, Patiala, PB, 147002, India

    Geetika Sirhindi & Poonam Sharma

  2. CSIR-Institute of Himalayan Bioresource Technology, Palampur, HP, 176061, India

    Preeti Arya, Parul Goel, Gulshan Kumar, Vishal Acharya & Anil Kumar Singh

  3. Academy of Scientific and Innovative Research, New Delhi, India

    Preeti Arya, Parul Goel, Gulshan Kumar, Vishal Acharya & Anil Kumar Singh

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  1. Geetika Sirhindi
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  2. Poonam Sharma
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Correspondence to Anil Kumar Singh.

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Sirhindi, G., Sharma, P., Arya, P. et al. Genome-wide characterization and expression profiling of TIFY gene family in pigeonpea (Cajanus cajan (L.) Millsp.) under copper stress. J. Plant Biochem. Biotechnol. 25, 301–310 (2016). https://doi.org/10.1007/s13562-015-0342-6

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