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Anatolıan sage Salvıa frutıcosa ınhıbıts cytosolıc glutathıone-s-transferase actıvıty and colon cancer cell prolıferatıon

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Abstract

Colorectal cancer is one of the leading causes of death worldwide, and healthy diet is critical in preventing it. This study intends to investigate phenolic profile, antioxidant, and anti-proliferative activity as well as Glutathione S-transferase (GST) inhibition activity of water extract of Salvia fruticosa Mill. (SF.) HPLC analysis revealed that the main bioactive ingredients of SF were rosmarinic and caffeic acid. Total phenolic and flavonoid contents were 425.1 mg GAE/g extract and 94.7 mg CE/g extract, respectively. SF exhibited high antioxidant activity through DPPH and ABTS assays. The extract exhibited time and dose-dependent anti-proliferative activity on colorectal adenocarcinoma cell lines (Caco-2 and HT-29). The extract also inhibited GST activity in a mixed-type manner, with an IC50 value of 6.8 µg/mL. To conclude, the results suggest that SF may be considered as a medicinal plant which can be used in theprevention and treatment of colon cancer, besides chemotherapy drugs.

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Authors and Affiliations

  1. Department of Chemistry, Erzincan University, 24100, Erzincan, Turkey

    Ahmet Altay

  2. Biochemistry Department, Middle East Technical University, 06800, Ankara, Turkey

    Ahmet Altay

  3. Faculty of Science, Department of Biology, Hacettepe University, 06800, Beytepe, Ankara, Turkey

    Aysun Kılıc Suloglu & Guldenız Selmanoglu

  4. Faculty of Health Sciences, Nutrition and Dietetics Department, Nutritional Sciences Division, Gazi University, 06560, Besevler, Ankara, Turkey

    Gulcın Sagdıcoglu Celep

  5. Department of Gastronomy and Kitchen Arts, Dogus University, 34722, Acıbadem, Istanbul, Kadikoy, Turkey

    Gulcın Sagdıcoglu Celep & Faruk Bozoglu

  6. Food Engineering Department, Middle East Technical University, 06800, Ankara, Turkey

    Gulcın Sagdıcoglu Celep

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  1. Ahmet Altay
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Altay, A., Kılıc Suloglu, A., Sagdıcoglu Celep, G. et al. Anatolıan sage Salvıa frutıcosa ınhıbıts cytosolıc glutathıone-s-transferase actıvıty and colon cancer cell prolıferatıon. Food Measure 13, 1390–1399 (2019). https://doi.org/10.1007/s11694-019-00055-1

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