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Differential Proteomics Analysis of Endometriosis in Blood Stasis Syndrome

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Abstract

Objective

To investigate the innate characters of 3 endometriosis (EMT) syndromes, blood stasis (BS), qi stagnation and blood stasis (QSBS) as well as Shen (Kidney) deficiency and blood stasis (KDBS) in terms of proteomics, lay a molecular biological basis for the differentiation of various blood stasis syndromes of EMT, establish a EMT microscopic syndrome differentiation and diagnosis system in terms of proteomics, discover the evolution principles and therapeutic targets of these EMT syndromes, and search their signifificant molecular markers and genetic intervention targets.

Methods

Six specimens from the ectopic and entopic endometrium tissues of patients with EMT in each syndrome, BS, QSBS as well as KDBS, in the early proliferative phase of the menstrual cycle, and 6 specimens from normal endometrium tissues in the early proliferative phase of the menstrual cycle were obtained. Three groups were formed in each syndrome by mixing two random specimens in equal amount, and then their respective two-dimensional electrophoresis graphs were obtained after total protein extraction. Finally, the detected differences in protein expression were identifified through matrix-assisted laser desorption Ionization-time of flflight mass spectrometry (MALDI-TOF/MS) and protein database.

Results

The results of differential proteins expressed in each syndrome were shown as follows: BS syndrome had 2 differential proteins in entopic endometrium and 1 differential protein in ectopic endometrium; KDBS syndrome had 3 in entopic endometrium and 3 in ectopic endometrium; and QSBS syndrome had 3 in entopic endometrium and 4 in ectopic endometrium. It was found out that annexin was highly expressed in both entopic and ectopic endometrium of KDBS syndrome; and myosin light chain 3 was highly expressed in both entopic and ectopic endometrium of QSBS syndrome.

Conclusion

There are differential protein expressions among the 3 EMT syndromes, which might be the inner origin of syndrome characters, and these differential proteins might be the candidate biomarkers for the pathogenesis of various EMT syndromes.

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Authors and Affiliations

  1. Gynecology Department of Traditional Chinese Medicine, Clinical Medical College, Chengdu University of Traditional Chinese Medicine, Chengdu, 610075, China

    Yi Wen, Yan Wang, Ting-ting Feng & Shao-bin Wei

Authors
  1. Yi Wen
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  2. Yan Wang
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  3. Ting-ting Feng
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  4. Shao-bin Wei
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Corresponding author

Correspondence to Shao-bin Wei.

Additional information

Supported by National Natural Science Foundation of China (No. 30973770)

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Wen, Y., Wang, Y., Feng, Tt. et al. Differential Proteomics Analysis of Endometriosis in Blood Stasis Syndrome. Chin. J. Integr. Med. 24, 925–929 (2018). https://doi.org/10.1007/s11655-017-2401-4

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  • DOI: https://doi.org/10.1007/s11655-017-2401-4

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