Skip to main content
SpringerLink
Log in

Valinomycin, produced by Streptomyces sp. S8, a key antifungal metabolite in large patch disease suppressiveness

  • Original Paper
  • Published:
World Journal of Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

Large patch disease, caused by Rhizoctonia solani AG2-2, is the most devastating disease in Zoysiagrass (Zoysia japonica). Current large patch disease control strategies rely primarily upon the use of chemical pesticides. Streptomyces sp. S8 is known to possess exceptional antagonistic properties that could potentially suppress the large patch pathogen found at turfgrass plantations. This study aims to demonstrate the feasibility of using the strain as a biological control mechanism. Sequencing of the S8 strain genome revealed a valinomycin biosynthesis gene cluster. This cluster is composed of the vlm1 and vlm2 genes, which are known to produce antifungal compounds. In order to verify this finding for the large patch pathogen, a valinomycin biosynthesis knockout mutant was created via the CRISPR/Cas9 system. The mutant lost antifungal activity against the large patch pathogen. Consequently, it is anticipated that eco-friendly microbial preparations derived from the S8 strain can be utilized to biologically control large patch disease.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • Asano T, Senda M, Suga H, Kageyama K (2010) Development of multiplex PCR to detect five Pythium species related to turfgrass diseases. J Phytopathol 158:609–615

    CAS  Google Scholar 

  • Aziz RK, Bartels D, Best AA, DeJongh M, Disz T, Edwards RA, Meyer F (2008) The RAST server: rapid annotations using subsystems technology. BMC Genomics 9:75

    Article  Google Scholar 

  • Beard JB (1972) Turfgrass: science and culture. Prentice-Hall, Englewood Cliffs

    Google Scholar 

  • Bérdy J (2012) Thoughts and facts about antibiotics: where we are now and where we are heading. J Antibiot 65:385–395

    Article  Google Scholar 

  • Blin K, Medema MH, Kazempour D, Fischbach MA, Breitling R, Takano E, Weber T (2013) antiSMASH 2.0—a versatile platform for genome mining of secondary metabolite producers. Nucleic Acids Res 41:W204–W212

    Article  Google Scholar 

  • Breukink E, Wiedemann I, van Kraaij C, Kuipers OP, Sahl HG, de Kruijff B (1999) Use of the cell wall precursor lipid II by a pore-forming peptide antibiotic. Science 286:2361–2364

    Article  CAS  Google Scholar 

  • Cheng YQ (2006) Deciphering the biosynthetic codes for the potent anti-SARS-coV cyclodepsipeptide valinomycin in Streptomyces tsusimaensis ATCC 15141. Chem Bio Chem 7:471–477

    Article  CAS  Google Scholar 

  • Čihák M, Kameník Z, Šmídová K, Bergman N, Benada O, Kofroňová O, Petříčková K, Bobek J (2017) Secondary metabolites produced during the germination of Streptomyces coelicolor. Front Microbiol 13:2495

    Article  Google Scholar 

  • Cobb RE, Wang Y, Zhao H (2014) High-efficiency multiplex genome editing of Streptomyces species using an engineered CRISPR/Cas system. ACS Synth Biol 4:723–728

    Article  Google Scholar 

  • Couch HB, Lucas LT, Haygood RA (1990) The nature and control of Rhizoctonia blight. Golf Course Manag 58:49–58

    Google Scholar 

  • Delauney AJ, Verma DP (1993) Proline biosynthesis and osmoregulation in plants. Plant J 4:215–223

    Article  CAS  Google Scholar 

  • Engelke MC, Murray JJ, Yeam DY (1983) Distribution, collection and use of zoysiagrass in the Far East, part II. Agronomy Abstract, pp. 123–125

  • Gerhardson B (2002) Biological substitutes for pesticides. Trends Biotechnol 20:338–343

    Article  CAS  Google Scholar 

  • Graham J, Marshall B, Squire G (2003) Genetic differentiation over a spatial environmental gradient in wild Rubus ideaus populations. New Phytol 157:667–675

    Article  Google Scholar 

  • Hwang YS, Choi JS (1999) Effect of mowing interval, aeration, and fertility level on the turf quality and growth of zoysiagrass (Zoysia japonica Steud.). Kor Turfgrass Sci 13:79–90

    Google Scholar 

  • Islam MR, Jeong YT, Ryu YJ, Song CH, Lee YS (2009) Isolation, identification and optimal culture conditions of Streptomyces albidoflavus C247 producing antifungal agents against Rhizoctonia solani AG2-2. Mycobiology 37:114–120

    Article  CAS  Google Scholar 

  • Kieser T (2000) Practical Streptomyces genetics. John Innes Foundation, Norwich

    Google Scholar 

  • Lee DW, Lee SM, Kim DS, Choi TH, Chang TH (2012) Application of paraffin oil for control of large patch on Zoysia japonica. Asian J Turfgrass Sci 26:17–23

    CAS  Google Scholar 

  • Lee SM, Kim DS, Lee K, Lee C, Lee DW (2013) Antibiotic properties of Helicosporium sp. KCTC 0635BP to Rhizoctonia solani AG2-2 IV. Weed Turfgrass Sci 2:202–206

    Article  Google Scholar 

  • Lee JH, Min GY, Jeon CW, Choi SM, Shim GY, Kwak YS (2015a) Investigation of fungicide response of Streptomyces spp. isolated from rhizosphere in zoysiagrass. Kor J Pestic Sci 19:54–63

    Article  Google Scholar 

  • Lee JH, Min GY, Shim GY, Jeon CW, Kwak YS (2015b) Physiological characteristics of Actinomycetes isolated from turfgrass rhizosphere. Weed Turfgrass Sci 4:348–359

    Article  Google Scholar 

  • Lee JH, Min GY, Shim GY, Jeon CW, Choi SM, Han JJ, Kwak YS (2015c) Soil microbial community analysis in large patch (Rhizoctonia solani AG2-2 IV). Weed Turfgrass Sci 4:124–128

    Article  Google Scholar 

  • Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods 25:402–408

    Article  CAS  Google Scholar 

  • Matter AM, Hoot SB, Anderson PD, Neves SS, Cheng YQ (2009) Valinomycin biosynthetic gene cluster in Streptomyces: conservation, ecology and evolution. PLoS ONE 4:e7194

    Article  Google Scholar 

  • McCarty LB, Gregg MF, Toler JE, Camberato JJ, Hill HS (2005) Minimizing thatch and mat development in a newly seeded creeping bentgrass golf green. Crop Sci 45:1529–1535

    Article  Google Scholar 

  • Miller CS, Handley KM, Wrighton KC, Frischkorn KR, Thomas BC, Banfield JF (2013) Short-read assembly of full-length 16S amplicons reveals bacterial diversity in subsurface sediments. PLoS ONE 8:e56018

    Article  CAS  Google Scholar 

  • Monakhova OF, Chernyad'ev II (2002) Protective role of kartolin-4 in wheat plants exposed to soil draught. Appl Biochem Microbiol 38:373–380

    Article  CAS  Google Scholar 

  • Nawani NN, Kapadnis BP, Das AD, Rao AS, Mahajan SK (2002) Purification and characterization of a thermophilic and acidophilic chitinase from Microbispora sp. V2. J Appl Microbiol 93:965–975

    Article  CAS  Google Scholar 

  • Obasa K, Fry J, Kennelly M (2012) Susceptibility of zoysiagrass germplasm to large patch caused by Rhizoctonia solani. HortSci 47:1252–1256

    Article  Google Scholar 

  • Pan X, Richardson MD, Deng S, Kremer RJ, English JT, Mihail JD, Xiong X (2017) Effect of organic amendment and cultural practice on large patch occurrence and soil microbial community. Crop Sci 57:2263–2272

    Article  CAS  Google Scholar 

  • Park CN, Lee JM, Lee D, Kim BS (2008) Antifungal activity of valinomycin, a peptide antibiotic produced by Streptomyces sp. Strain M10 antagonistic to Botrytis cinerea. J Microbiol Biotechnol 18:880–884

    CAS  PubMed  Google Scholar 

  • Patton AJ, Schwartz BM, Kenworthy KE (2017) Zoysiagrass (Zoysia spp.) history, utilization, and improvement in the United States: a review. Crop Sci 57:S-37–S-72

    Article  Google Scholar 

  • Raaijmakers JM, Weller DM (1998) Natural plant protection by 2, 4-diacetylphloroglucinol-producing Pseudomonas spp. in take-all decline soils. Mol Plant Microbe Interact 11:144–152

    Article  CAS  Google Scholar 

  • Smiley RW, Dernoeden PH, Clarke BB (2005) Compendium of turfgrass diseases, 3rd edn. American Phytopathological Society, St. Paul

    Book  Google Scholar 

  • Smirnoff N, Cumbes QJ (1989) Hydroxyl radical scavenging activity of compatible solutes. Phytochemistry 28:1057–1060

    Article  CAS  Google Scholar 

  • Song CH, Islam M, Chang TH, Lee YS (2012) Isolation and identification of antagonistic bacteria for biological control of large patch disease of zoysiagrass caused by Rhizoctonia solani AG2-2 (IV). Asian J Turfgrass Sci 26:8–16

    Google Scholar 

  • Suslov TV (1982) Role of root-colonizing bacteria in plant growth. In: Mount MS, Lacy GH (eds) Prokariotes phytopathogenic. Academic Press, London, pp 187–223

    Chapter  Google Scholar 

  • van Dessel W, van Mellaert L, Geukens N, Lammertyn E, Anné J (2004) Isolation of high quality RNA from Streptomyces. J Microbial Methods 58:135–137

    Article  Google Scholar 

  • Welbaum GE, Sturz AV, Dong Z, Nowak J (2004) Managing soil microorganisms to improve productivity of agro-ecosystems. Crit Rev Plant Sci 23:175–193

    Article  CAS  Google Scholar 

Download references

Acknowledgement

This work was supported by the Technology Development Program for Agriculture and Forestry, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea (Project No. 315004-5).

Author information

Author notes

  1. Chang-Wook Jeon and Da-Ran Kim have contributed equally to this work.

Authors and Affiliations

  1. Dvision of Applied Life Science (BK21Plus), Gyeongsang National University, Jinju, 52828, South Korea

    Chang-Wook Jeon & Youn-Sig Kwak

  2. Department of Plant Medicine and Institute of Agriculture & Life Sciences, Gyeongsang National University, Jinju, 52828, South Korea

    Da-Ran Kim & Youn-Sig Kwak

Authors
  1. Chang-Wook Jeon
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Da-Ran Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Youn-Sig Kwak
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Youn-Sig Kwak.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 24 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Jeon, CW., Kim, DR. & Kwak, YS. Valinomycin, produced by Streptomyces sp. S8, a key antifungal metabolite in large patch disease suppressiveness. World J Microbiol Biotechnol 35, 128 (2019). https://doi.org/10.1007/s11274-019-2704-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s11274-019-2704-z

Keywords

Search

Navigation