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Identification and characterization of the major antifungal substance against Fusarium Sporotrichioides from Chaetomium globosum

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Abstract

Fusarium sporotrichioides, is a common soil-borne plant pathogen causing dry rot of potato in Northeast China. The objective of this study was to identify the main antifungal substances from Chaetomium globosum W7 against F. sporotrichioides. Strain W7 can significantly inhibit F. sporotrichioides without direct contact, suggesting that its antifungal substance was extracellular, and the solubility of this antifungal substance in ethyl acetate was superior to that in water. Acetone was selected as the optimum solvent for the extraction of the metabolites of C. globosum. Metabolites were then separated with thin-layer chromatography. Following antifungal tests on bands, a dark brown band with Rf value of 0.20 was determined as the antifungal substance, and identified as chaetoglobosin A. The antifungal activity test showed that the minimum inhibitory concentration of chaetoglobosin A to F. sporotrichioides was 9.45–10.50 μg/mL, IC50 being 4.344 μg/mL. Chaetoglobosin A also proved to have an excellent preventive effect on potato dry rot caused by F. sporotrichioides. To summarize, chaetoglobosin A was identified as the main active substance of C. globosum to inhibit F. sporotrichioides for the first time, and demonstrated a potential application value in agriculture.

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References

  • Alabouvette C (1986) Fusarium-wilt suppressive soils from the Chateaurenard region—review of a 10-year study. Agronomie 6:273–284. doi:10.1051/agro:19860307

    Article  Google Scholar 

  • Beer SV, Rundle JR, Norelli JL (1984) Recent progress in the development of biological control for fire blight—a review. Acta Hortic 151:195–202. doi:10.17660/ActaHortic.1984.151.24

    Article  Google Scholar 

  • Chouvenc T, Su NY, Grace JK (2011) Fifty years of attempted biological control of termites—analysis of a failure. Biol Control 59:69–82. doi:10.1016/j.biocontrol.2011.06.015

    Article  Google Scholar 

  • CLSI (2008) Reference method for broth dilution antifungal susceptibility testing of filamentous fungi; approved standard-second edtion. CLSI document M38-A2. vol 28.. Clinical and Laboratory Standards Institute, Wayne, PA

    Google Scholar 

  • Di PA, Gutrella M, Pachlatko JP, Schwinn FJ (1992) Role of antibiotics produced by Chaetomium globosum in biocontrol of Pythium ultimum, a causal agent of damping-off. Phytopathology 82:131–135

    Article  Google Scholar 

  • Hu Y, Hao X, Lou J, Zhang P, Pan J, Zhu X (2012) A PKS gene, pks-1, is involved in chaetoglobosin biosynthesis, pigmentation and sporulation in Chaetomium globosum. Sci China-Life Sci 55:1100–1108. doi:10.1007/s11427-012-4409-5

    Article  CAS  Google Scholar 

  • Hu Y, Zhang W, Zhang P, Ruan W, Zhu X (2013) Nematicidal activity of chaetoglobosin A poduced by Chaetomium globosum NK102 against Meloidogyne incognita. J Agric Food Chem 61:41–46. doi:10.1021/jf304314g

    Article  CAS  Google Scholar 

  • Ichihara A, Katayama K, Teshima H, Oikawa H, Sakamura S (1996) Chaetoglobosin O and other phytotoxic metabolites from Cylindrocladium floridanum, a causal fungus of alfalfa black rot disease. Biosci Biotechnol Biochem 60:360–361. doi:10.1271/bbb.60.360

    Article  CAS  Google Scholar 

  • Ishiuchi K, Nakazawa T, Yagishita F, Mino T, Noguchi H, Hotta K, Watanabe K (2013) Combinatorial generation of complexity by redox enzymes in the chaetoglobosin A biosynthesis. J Am Chem Soc 135:7371–7377. doi:10.1021/ja402828w

    Article  CAS  Google Scholar 

  • Jiang C, Song J, Cong H, Zhang J, Yang Q (2016) Expression and characterization of a novel antifungal exo-β-1,3-glucanase from Chaetomium cupreum. Appl Biochem Biotechnol. doi:10.1007/s12010-016-2325-z

    Google Scholar 

  • Jiang C, Song J, Zhang J, Yang Q (2017) New production process of the antifungal chaetoglobosin A using cornstalks. Braz J Microbiol. doi:10.1016/j.bjm.2016.11.008

    Google Scholar 

  • Jiao W, Feng Y, Blunt JW, Cole AL, Munro MH (2004) Chaetoglobosins Q, R, and T, three further new metabolites from Chaetomium globosum. J Nat Prod 67:1722–1725. doi:10.1021/np030460g

    Article  CAS  Google Scholar 

  • Knudsen PB et al (2014) Chaetoglobosin A preferentially induces apoptosis in chronic lymphocytic leukemia cells by targeting the cytoskeleton. Leukemia 28:1289–1298. doi:10.1038/leu.2013.360

    Article  CAS  Google Scholar 

  • Kotan R, Sahin F, Demirci E, Eken C (2009) Biological control of the potato dry rot caused by Fusarium species using PGPR strains. Biol Control 50:194–198. doi:10.1016/j.biocontrol.2009.04.004

    Article  Google Scholar 

  • Kwjr S, Datnoff LE, Correavictoria FJ, Kucharek TA, Snyder GH (2004) Effect of silicon and fungicides on the control of leaf and neck blast in upland rice. Plant Dis 88:253–258. doi:10.1094/PDIS.2004.88.3.253

    Article  Google Scholar 

  • Li Q et al (2011) Suppression of Magnaporthe oryzae by culture filtrates of Streptomyces globisporus JK-1. Biol Control 58:139–148. doi:10.1016/j.biocontrol.2011.04.013

    Article  CAS  Google Scholar 

  • Raaijmakers JM, Leeman M, Mmpvan O, Ivander S, Schippers B, Pahm B (1995) Dose-response relationships in biological control of Fusarium wilt of radish by Pseudomonas spp. Phytopathology 85:1075–1081

    Article  Google Scholar 

  • Rojo FG, Reynoso MM, Ferez M, Chulze SN, Torres AM (2007) Biological control by Trichoderma species of Fusarium solani causing peanut brown root rot under field conditions. Crop Prot 26:549–555. doi:10.1016/j.cropro.2006.05.006

    Article  Google Scholar 

  • Scherlach K, Boettger D, Remme N, Hertweck C (2010) The chemistry and biology of cytochalasans. Nat Prod Rep 27:869–886. doi:10.1039/B903913A

    Article  CAS  Google Scholar 

  • Sekita S, Yoshihira K, Natori S, Kuwano H (1973) Structures of chaetoglobosin A and B, cytotoxic metabolites of Chaetomium globosum. Tetrahedron Lett 14:2109–2112. doi:10.1016/S0040-4039(01)86820-9

    Article  Google Scholar 

  • Sekita S, Yoshihira K, Natori S, Udagawa S, Sakabe F, Kurata H, Umeda M (1982) Chaetoglobosins, cytotoxic 10-(indol-3-yl)-[13]cytochalasans from Chaetomium spp. I. Production, isolation and some cytological effects of chaetoglobosins A-J. Chem Pharm Bull 30:1609–1617. doi:10.1248/cpb.30.1609

    Article  CAS  Google Scholar 

  • Sibounnavong P, Charoenporn C, Kanokmedhakul S, Soytong K (2011) Antifungal metabolites from antagonistic fungi used to control tomato wilt fungus Fusarium oxysporum f. sp. lycopersici. Afr J Biotechnol 10:19714–19722. doi:10.5897/Ajb11.3343

    Article  Google Scholar 

  • Soytong K, Kanokmedhakuf S, Kanokmedhakul S, Kukongviriyapa V (2001) Application of Chaetomium species (Ketomium R) as a new broad spectrum biological fungicide for plant disease control. Fungal Divers 7:1–15

    Google Scholar 

  • Soytong K, Srinon W, Rattanacherdchai K, Kanokmedhakul S, Kanokmedhakul K (2005) Application of antagonistic fungi to control anthracnose disease of grape. J Agric Technol 1:33–41

    Google Scholar 

  • Tekauz A, McCallum B, Gilbert J (2009) Review: Fusarium head blight of barley in western Canada. Can J Plant Pathol 22:9–16. doi:10.1080/07060660009501156

    Article  Google Scholar 

  • Tveit M, Wood RKS (2010) The control of Fusarium blight in oat seedlings with antagonistic species of Chaetomium. Ann Appl Biol 43:538–552. doi:10.1111/j.1744-7348.1955.tb02503.x

    Article  Google Scholar 

  • Wang YJ, Yang Q (2009) Cloning and expression of a novel chitinase chi58 from Chaetomium cupreum in Pichia pastoris. Biochem Genet 47:547–558. doi:10.1007/s10528-009-9251-5

    Article  CAS  Google Scholar 

  • Yuan J, Raza W, Shen Q, Huang Q (2012) Antifungal activity of Bacillus amyloliquefaciens NJN-6 volatile compounds against Fusarium oxysporum f. sp. cubense. Appl Environ Microbiol 78:5942–5944. doi:10.1128/AEM.01357-12

    Article  CAS  Google Scholar 

  • Zhang Q, Li HQ, Zong SC, Gao JM, Zhang AL (2012) Chemical and bioactive diversities of the genus Chaetomium secondary metabolites. Mini Rev Med Chem 12:127. doi:10.2174/138955712798995066

    Article  Google Scholar 

  • Zhang G et al (2013a) Efficacy assessment of antifungal metabolites from Chaetomium globosum No.05, a new biocontrol agent, against Setosphaeria turcica. Biol Control 64:90–98. doi:10.1016/j.biocontrol.2012.10.005

    Article  CAS  Google Scholar 

  • Zhang G, Zhang Y, Qin J, Qu X, Liu J, Li X, Pan H (2013b) Antifungal metabolites produced by Chaetomium globosum No.04, an endophytic fungus Isolated from Ginkgo biloba. Indian J Microbiol 53:175–180. doi:10.1007/s12088-013-0362-7

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors wish to thank the Chinese government for the financial support of this study under the National High Technology Research and Development Program (2011AA10A205) and “Twelfth Five-Year Plan” National Science and Technology Program on Rural Area (2014BAL02B00).

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Authors and Affiliations

  1. School of Life Sciences and Technology, Harbin Institute of Technology, Harbin, Heilongjiang, People’s Republic of China

    Cheng Jiang, Jinzhu Song, Junzheng Zhang & Qian Yang

  2. School of Chemical Engineering and Technology, Harbin Institute of Technology, Harbin, Heilongjiang, People’s Republic of China

    Junzheng Zhang

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  1. Cheng Jiang
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  2. Jinzhu Song
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  4. Qian Yang
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Correspondence to Qian Yang.

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Jiang, C., Song, J., Zhang, J. et al. Identification and characterization of the major antifungal substance against Fusarium Sporotrichioides from Chaetomium globosum . World J Microbiol Biotechnol 33, 108 (2017). https://doi.org/10.1007/s11274-017-2274-x

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