Abstract
Antimicrobial peptides have captured the attention of researchers in recent years because of their efficiency in fighting against pathogens. These peptides are found in nature and have been isolated from a wide range of organisms. Furthermore, analogs or synthetic derivatives have successfully been developed on the basis of natural peptide patterns. Long use of pesticides and antibiotics has led to development of resistance among pathogens and other pests as well as increase of environmental and health risks. Antimicrobial peptides are under consideration as new substitutes for conventional pesticides and antibiotics. Many plants and animals have been manipulated with antimicrobial peptide-encoding genes and several pesticides and drugs have been produced based on these peptides. Such strategies and products may still have a long way to go before being confirmed by regulatory bodies and others need to surmount technical problems before being accepted as applicable ones. In spite of these facts, several cases of successful use of antimicrobial peptides in agriculture and food industry indicate a promising future for extensive application of these peptides. In this review, we consider the developing field of antimicrobial peptide applications in various agricultural activities.
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References
Akinbowale OL, Peng H, Barton MD (2006) Antimicrobial resistance in bacteria isolated from aquaculture sources in Australia. J Appl Microbiol 100:1103–1113. doi:10.1111/j.1365-2672.2006.02812.x
Alan AR, Earle ED (2002) Sensitivity of bacterial and fungal plant pathogens to the lytic peptides, MSI-99, magainin II, and cecropin B. Mol Plant Microbe Interact 15:701–708. doi:10.1094/MPMI.2002.15.7.701
Alan AR, Blowers A, Earle ED (2004) Expression of a magainin-type antimicrobial peptide gene (MSI-99) in tomato enhances resistance to bacterial speck disease. Plant Cell Rep 22:388–396. doi:10.1007/s00299-003-0702-x
Alexander MC (2005) Antimicrobial peptide microbicides targeting HIV. Protein Pept Lett 12:41–47. doi:10.2174/0929866053406101
Appendini P, Hotchkiss JH (2002) Review of antimicrobial food packaging. Innov Food Sci Emerg Technol 3:113–126. doi:10.1016/S1466-8564(02)00012-7
Arakawa T (2003) Chitin synthesis-inhibiting antifungal agents promote nucleopolyhedrovirus infection in silkworm, Bombyx mori (Lepidoptera: Bombycidae) larvae. J Invertebr Pathol 83:261–263. doi:10.1016/S0022-2011(03)00085-5
Bhargava A, Osusky M, Forward BS, Hancock RE, Kay WW, Misra S (2007) Expression of a polyphemusin variant in transgenic tobacco confers resistance against plant pathogenic bacteria, fungi and a virus. Plant Cell Tissue Organ Cult 88:301–312. doi:10.1007/s11240-007-9204-9
Brown KL, Hancock REW (2006) Cationic host defense (antimicrobial) peptides. Curr Opin Immunol 18:24–30. doi:10.1016/j.coi.2005.11.004
Chakrabarti A, Ganapathi TR, Mukherjee PK, Bapat VA (2003) MSI-99, a magainin analogue, imparts enhanced disease resistance in transgenic tobacco and banana. Planta 216:587–596
Chan JC, Li-Chan EC (2007) Production of lactoferricin and other cationic peptides from food grade bovine lactoferrin with various iron saturation levels. J Agric Food Chem 55:493–501. doi:10.1021/jf0625149
Chernysh S, Kim SI, Bekker G, Pleskach VA, Filatova NA, Anikin VB, Platonov VG, Bulet P (2002) Antiviral and antitumor peptides from insects. Proc Natl Acad Sci USA 99:12628–12632. doi:10.1073/pnas.192301899
Chiou PP, Lin CM, Perez L, Chen TT (2002) Effect of cecropin B and a synthetic analogue on propagation of fish viruses in vitro. Mar Biotechnol 4:294–302. doi:10.1007/s10126-002-0021-1
Coca M, Penas G, Gomez J, Campo S, Bortolotti C, Messeguer J, Segundo BS (2006) Enhanced resistance to the rice blast fungus Magnaporthe grisea conferred by expression of a cecropin A gene in transgenic rice. Planta 223:392–406. doi:10.1007/s00425-005-0069-z
Conlon JM, Al-Ghaferi N, Abraham B, Leprince J (2007) Strategies for transformation of naturally-occurring amphibian antimicrobial peptides into therapeutically valuable anti-infective agents. Methods 42:349–357. doi:10.1016/j.ymeth.2007.01.004
De Lucca AJ, Walsh TJ (2000) Antifungal peptides: origin, activity, and therapeutic potential. Rev Iberoam Micol 17:116–120
DeGray G, Rajasekaran K, Smith F, Sanford J, Daniell H (2001) Expression of an antimicrobial peptide via the chloroplast genome to control phytopathogenic bacteria and fungi. Plant Physiol 127:852–862. doi:10.1104/pp.010233
Delves-Broughton J (2005) Nisin as a food preservative. Food Aust 57:525–527
Donovan DM, Kerr DE, Wall RJ (2005) Engineering disease resistant cattle. Transgenic Res 14:563–567. doi:10.1007/s11248-005-0670-8
Egorov TA, Odintsova TI, Pukhalsky VA, Grishin EV (2005) Diversity of wheat anti-microbial peptides. Peptides 26:2064–2073. doi:10.1016/j.peptides.2005.03.007
Fan W, Plaut K, Bramley AJ, Barlow JW, Kerr DE (2002) Adenoviral-mediated transfer of a lysostaphin gene into the goat mammary gland. J Dairy Sci 85:1709–1716
Florack DEA, Stiekema WJ, Bosch D (1996) Toxicity of peptides to bacteria present in the vase water of cut roses. Postharvest Biol Technol 8:285–291. doi:10.1016/0925-5214(96)00009-9
Franklin NB, Cooksey KD, Getty KJ (2004) Inhibition of Listeria monocytogenes on the surface of individually packaged hot dogs with a packaging film coating containing nisin. J Food Prot 67:480–485
Gallagher T, Shaffer B, Rummer B (2006) An economic analysis of hardwood fiber production on dryland irrigated sites in the US Southeast. Biomass Bioenergy 30:794–802. doi:10.1016/j.biombioe.2005.08.004
Gao AG, Hakimi SM, Mittanck CA, Wu Y, Woerner BM, Stark DM, Shah DM, Liang J, Rommens CM (2000) Fungal pathogen protection in potato by expression of a plant defensin peptide. Nat Biotechnol 18:1307–1310. doi:10.1038/82436
Gennadios A, Hanna MA, Kurth LB (1997) Application of edible coatings on meats, poultry and seafoods: a review. Lebenson Wiss Technol 30:337–350. doi:10.1006/fstl.1996.0202
Giangaspero A, Sandri L, Tossi A (2001) Amphipathic α helical antimicrobial peptides A systematic study of the effects of structural and physical properties on biological activity. Eur J Biochem 268:5589–5600. doi:10.1046/j.1432-1033.2001.02494.x
Gordon YJ, Romanowski EG, McDermott AM (2005) A review of antimicrobial peptides and their therapeutic potential as anti-infective drugs. Curr Eye Res 30:505–515. doi:10.1080/02713680590968637
Hancock REW (2005) Mechanisms of action of newer antibiotics for Gram-positive pathogens. Lancet Infect Dis 5:209–218. doi:10.1016/S1473-3099(05)70051-7
Hancock REW, Chapple DS (1999) Peptide antibiotics. Antimicrob Agents Chemother 43:1317–1323
Jang SH, Park Y, Park SC, Kim PI, Lee DG, Hahm KS (2004) Antinematodal activity and the mechanism of the antimicrobial peptide, HP (2–20), against Caenorhabditis elegans. Biotechnol Lett 26:287–291. doi:10.1023/B:BILE.0000015427.26410.d4
Janisiewicz WJ, Korsten L (2002) Biological control of postharvest diseases of fruits. Annu Rev Phytopathol 40:411–441. doi:10.1146/annurev.phyto.40.120401.130158
Jaynes JM, Nagpala P, Destefanobeltran L, Huang JH, Kim JH, Denny T, Centiner S (2002) Expression of a cecropin-B lytic peptide analog in transgenic tobacco confers enhanced resistance to bacterial wilt caused by Pseudomonas solanacearum. Plant Sci 89:43–53. doi:10.1016/0168-9452(93)90169-Z
Jia X, Patrzykat A, Devlin RH, Ackerman PA, Iwama GK, Hancock REW (2000) Antimicrobial peptides protect Coho salmon from Vibrio anguillarum infections. Appl Environ Microbiol 66:1928–1932. doi:10.1128/AEM.66.5.1928-1932.2000
Jones RW, Prusky D (2002) Expression of an antifungal peptide in Saccharomyces: a new approach for biological control of the postharvest disease caused by Colletotrichum coccodes. Phytopathology 92:33–37. doi:10.1094/PHYTO.2002.92.1.33
Jones RW, Ospina-Giraldo M, Clemente T (2004) Prosystemin-antimicrobial-peptide fusion reduces tomato late blight lesion expansion. Mol Breed 14:83–89. doi:10.1023/B:MOLB.0000038001.22029.07
Kamysz W, Okroj M, Lukasiak J (2003) Novel properties of antimicrobial peptides. Acta Biochim Pol 50:461–469
Kazan K, Rusu A, Marcus JP, Goulter KC, Manners JM (2002) Enhanced quantitative resistance to Leptosphaeria maculans conferred by expression of a novel antimicrobial peptide in canola (Brassica napus L.). Mol Breed 10:63–70. doi:10.1023/A:1020354809737
Kerr DE, Plaut K, Bramley AJ, Williamson CM, Lax AJ, Moore K, Wells KD, Wall RJ (2001) Lysostaphin expression in mammary glands confers protection against staphylococcal infection in transgenic mice. Nat Biotechnol 19:66–70. doi:10.1038/83540
Khachatourians GG (1998) Agricultural use of antibiotics and the evolution and transfer of antibiotic-resistant bacteria. Can Med Assoc J 159:1129–1136
Koo JC, Chun HJ, Park HC, Kim MC, Koo YD, Koo SC, Ok HM, Park SJ, Lee SH, Yun DJ, Lim CO, Bahk JD, Lee SY, Cho MJ (2002) Over-expression of a seed specific hevein-like antimicrobial peptide from Pharbitis nil enhances resistance to a fungal pathogen in transgenic tobacco plants. Plant Mol Biol 50:441–452. doi:10.1023/A:1019864222515
Lee DG, Shin SY, Hahm KS (2004) Structure and fungicidal activity of a synthetic antimicrobial peptide, P18, and its truncated peptides. Biotechnol Lett 26:337–341. doi:10.1023/B:BILE.0000015472.09542.6d
Le-Feuvre RR, Ramírez CC, Olea N, Meza-Basso L (2007) Effect of the antimicrobial peptide indolicidin on the green peach aphid Myzus persicae (Sulzer). J Appl Entomol 131:71–75
Li Q, Lawrence CB, Davies HM, Everett NP (2002) A tridecapeptide possesses both antimicrobial and protease-inhibitory activities. Peptides 23:1–6. doi:10.1016/S0196-9781(01)00572-1
Liang H, Catranis CM, Maynard CA, Powell WA (2002) Enhanced resistance to the poplar fungal pathogen, Septoria musiva, in hybrid poplar clones transformed with genes encoding antimicrobial peptides. Biotechnol Lett 24:383–389. doi:10.1023/A:1014552503140
Liu Z, Zeng M, Dong S, Xu J, Song H, Zhao Y (2007) Effect of an antifungal peptide from oyster enzymatic hydrolysates for control of gray mold (Botrytis cinerea) on harvested strawberries. Postharvest Biol Technol 46:95–98. doi:10.1016/j.postharvbio.2007.03.013
Lopez-Garcia B, Veyrat A, Perez-Paya E, Gonzalez-Candelas L, Marcos JF (2003) Comparison of the activity of antifungal hexapeptides and the fungicides thiabendazole and imazalil against postharvest fungal pathogens. Int J Food Microbiol 89:163–170. doi:10.1016/S0168-1605(03)00118-1
Lortal S, Chapot-Chartier MP (2005) Role, mechanisms and control of lactic acid bacteria lysis in cheese. Int Dairy J 15:857–871. doi:10.1016/j.idairyj.2004.08.024
Lu G (2003) Engineering Sclerotinia sclerotiorum resistance in oilseed crops. Afr J Biotechnol 2:509–516
Ma Z, Michailides TJ (2005) Advances in understanding molecular mechanisms of fungicide resistance and molecular detection of resistant genotypes in phytopathogenic fungi. Crop Prot 24:853–863. doi:10.1016/j.cropro.2005.01.011
Marcos JF, Beachy RN, Houghten RA, Blondelle SE, Perez-Paya E (1995) Inhibition of a plant virus infection by analogs of melittin. Proc Natl Acad Sci USA 92:12466–12469. doi:10.1073/pnas.92.26.12466
Marcos JF, Muñoz A, Pérez-Payá E, Misra S, López-García B (2008) Identification and rational design of novel antimicrobial peptides for plant protection. Annu Rev Phytopathol 46:273–301. doi:10.1146/annurev.phyto.121307.094843
Mentag R, Luckevich M, Morency MJ, Seguin A (2003) Bacterial disease resistance of transgenic hybrid poplar expressing the synthetic antimicrobial peptide D4E1. Tree Physiol 23:405–411
Molina A, Molina MP, Althaus RL, Gallego L (2003) Residue persistence in sheep milk following antibiotic therapy. Vet J 165:84–89. doi:10.1016/S1090-0233(02)00173-9
Mullins E, Milbourne D, Petti C, Doyle-Prestwich BM, Meade C (2006) Potato in the age of biotechnology. Trends Plant Sci 11:254–260. doi:10.1016/j.tplants.2006.03.002
Newhouse AE, Schrodt F, Liang H, Maynard CA, Powell WA (2007) Transgenic American elm shows reduced Dutch elm disease symptoms and normal mycorrhizal colonization. Plant Cell Rep 26:977–987. doi:10.1007/s00299-007-0313-z
Norelli JL, Borejsza-Wysocka E, Reynoird JP, Aldwinckle HS (2000) Transgenic ‘Royal Gala’ apple expressing attacin E has increased field resistance to Erwinia amylovora (fire blight). Acta Hortic 538:631–633
O’Callaghan M, Gerard EM, Waipara NW, Young SD, Glare TR, Barrell PJ, Conner AJ (2004) Microbial communities of Solanum tuberosum and magainin-producing transgenic lines. Plant Soil 266:47–56. doi:10.1007/s11104-005-3714-1
Oard SV, Enright FM (2006) Expression of the antimicrobial peptides in plants to control phytopathogenic bacteria and fungi. Plant Cell Rep 25:561–572. doi:10.1007/s00299-005-0102-5
Osusky M, Zhou G, Osuska L, Hancock RE, Kay WW, Misra S (2000) Transgenic plants expressing cationic peptide chimeras exhibit broad-spectrum resistance to phytopathogens. Nat Biotechnol 18:1162–1166. doi:10.1038/81145
Osusky M, Osuska L, Hancock RE, Kay WW, Misra S (2004) Transgenic potatoes expressing a novel cationic peptide are resistant to late blight and pink rot. Transgenic Res 13:181–190. doi:10.1023/B:TRAG.0000026076.72779.60
Osusky M, Osuska L, Kay W, Misra S (2005) Genetic modification of potato against microbial diseases: in vitro and in planta activity of a dermaseptin B1 derivative, MsrA2. Theor Appl Genet 111:711–722. doi:10.1007/s00122-005-2056-y
Oumer BA, Gaya P, Fernandez-Garcia E, Marciaca R, Garde S, Medina M, Nunez M (2001) Proteolysis and formation of volatile compounds in cheese manufactured with a bacteriocin-producing adjunct culture. J Dairy Res 68:117–129. doi:10.1017/S0022029900004568
Park Y, Jang SH, Lee DG, Hahm KS (2004) Antinematodal effect of antimicrobial peptide, PMAP-23, isolated from porcine myeloid against Caenorhabditis elegans. J Pept Sci 10:304–311. doi:10.1002/psc.518
Pawar DD, Malik SVS, Bhilegaonkar KN, Barbuddhe SB (2000) Effect of nisin and its combination with sodium chloride on the survival of Listeria monocytogenes added to raw buffalo meat mince. Meat Sci 56:215–219. doi:10.1016/S0309-1740(00)00043-7
Perron GG, Zasloff M, Bell G (2006) Experimental evolution of resistance to an antimicrobial peptide. Proc Biol Sci 273:251–256. doi:10.1098/rspb.2005.3301
Peters RJ (2006) Uncovering the complex metabolic network underlying diterpenoid phytoalexin biosynthesis in rice and other cereal crop plants. Phytochemistry 67:2307–2317. doi:10.1016/j.phytochem.2006.08.009
Ponti D, Mangoni ML, Mignogna G, Simmaco M, Barra D (2003) An amphibian antimicrobial peptide variant expressed in Nicotiana tabacum confers resistance to phytopathogens. Biochem J 370:121–127. doi:10.1042/BJ20021444
Powers JS, Hancock REW (2003) The relationship between peptide structure and antibacterial activity. Peptides 24:1681–1691. doi:10.1016/j.peptides.2003.08.023
Reed WA, Elzer PH, Enright FM, Jaynes JM, Morrey JD, White KL (1997) Interleukin 2 promoter/enhancer controlled expression of a synthetic cecropin-class lytic peptide in transgenic mice and subsequent resistance to Brucella abortus. Transgenic Res 6:337–347. doi:10.1023/A:1018423015014
Rekha, Naik SN, Prasad R (2006) Pesticide residue in organic and conventional food-risk analysis. J Chem Health Saf 13:12–19
Reuveni M, Cohen H, Zahavi T, Venezian A (2000) Polar-a potent Polyoxin B compound for controlling powdery mildews in apple and nectarine trees, and grapevines. Crop Prot 19:393–399. doi:10.1016/S0261-2194(00)00030-2
Reynoird JP, Mourgues F, Norelli J, Aldwinckle HS, Brisset MN, Chevreau E (1999) First evidence for improved resistance to fire blight in transgenic pear expressing the attacin E gene from Hyalophora cecropia. Plant Sci 149:23–31. doi:10.1016/S0168-9452(99)00139-9
Rommens CM (2004) All-native DNA transformation: a new approach to plant genetic engineering. Trends Plant Sci 9:457–464. doi:10.1016/j.tplants.2004.07.001
Rydlo T, Miltz J, Mor A (2006) Eukaryotic antimicrobial peptides: promises and premises in food safety. J Food Sci 71:125–135. doi:10.1111/j.1750-3841.2006.00175.x
Sarmasik A, Warr G, Chen TT (2002) Production of transgenic medaka with increased resistance to bacterial pathogens. Mar Biotechnol 4:310–322. doi:10.1007/s10126-002-0023-z
Schaefer SC, Gasic K, Cammue B, Broekaert W, van Damme EJ, Peumans WJ, Korban SS (2005) Enhanced resistance to early blight in transgenic tomato lines expressing heterologous plant defense genes. Planta 222:858–866. doi:10.1007/s00425-005-0026-x
Schröder JM, Harder J (2006) Antimicrobial peptides in skin disease. Drug Discov Today Ther Strateg 3:93–100. doi:10.1016/j.ddstr.2006.02.007
Sen AK, Narbad A, Horn N, Dodd HM, Parr AJ, Colquhoun I, Gasson MJ (1999) Post-translational modification of nisin. The involvement of NisB in the dehydration process. Eur J Biochem 261:524–532. doi:10.1046/j.1432-1327.1999.00303.x
Sharma A, Sharma R, Imamura M, Yamakawa M, Machii H (2000) Transgenic expression of cecropin B, an antibacterial peptide from Bombyx mori, confers enhanced resistance to bacterial leaf blight in rice. FEBS Lett 484:7–11. doi:10.1016/S0014-5793(00)02106-2
Soltani S, Keymanesh K, Sardari S (2007) In silico analysis of antifungal peptides: determining the lead template sequence of potent antifungal peptides. Expert Opin Drug Discov 2:1–11. doi:10.1517/17460441.2.6.837
Tripathi L (2003) Genetic engineering for improvement of Musa production in Africa. Afr J Biotechnol 2:503–508
van der Biezen EA (2001) Quest for antimicrobial genes to engineer disease-resistant crops. Trends Plant Sci 6:89–91. doi:10.1016/S1360-1385(01)01870-2
Venkitanarayanan KS, Zhao T, Doyle MP (1999) Antibacterial effect of lactoferricin B on Escherichia coli O157:H7 in ground beef. J Food Prot 62:747–750
Vidal JR, Kikkert JR, Malnoy MA, Wallace PG, Barnard J, Reisch BI (2006) Evaluation of transgenic ‘Chardonnay’ (Vitis vinifera) containing magainin genes for resistance to crown gall and powdery mildew. Transgenic Res 15:69–82. doi:10.1007/s00299-003-0682-x
Vizioli J, Salzet M (2002a) Antimicrobial peptides versus parasitic infections? Trends Parasitol 18:475–476. doi:10.1016/S1471-4922(02)02428-5
Vizioli J, Salzet M (2002b) Antimicrobial peptides from animals: focus on invertebrates. Trends Pharmacol Sci 23:494–496. doi:10.1016/S0165-6147(02)02105-3
Wang HX, Ng TB (2005) An antifungal peptide from the coconut. Peptides 26:2392–2396. doi:10.1016/j.peptides.2005.05.009
Wang Y, Nowak G, Culley D, Hadwiger LA, Fristensky B (1999) Constitutive expression of pea defense gene DRR206 confers resistance to blackleg (Leptosphaeria maculans) disease in transgenic canola (Brassica napus). Mol Plant Microbe Interact 12:410–418. doi:10.1094/MPMI.1999.12.5.410
Wang JX, Zhao XF, Liang YL, Li L, Zhang W, Ren Q, Wang LC, Wang LY (2006) Molecular characterization and expression of the antimicrobial peptide defensin from the housefly (Musca domestica). Cell Mol Life Sci 63:3072–3082. doi:10.1007/s00018-006-6284-3
Webb CA, Fellers JP (2004) Cereal rust fungi genomics and the pursuit of virulence and avirulence factors. FEMS Microbiol Lett 264:1–7. doi:10.1111/j.1574-6968.2006.00400.x
Weinberg ED (2003) The therapeutic potential of lactoferrin. Expert Opin Investig Drugs 12:841–851. doi:10.1517/13543784.12.5.841
Wisniewski ME, Bassett CL, Artlip TS, Janisiewicz WJ, Norelli JL, Droby S (2005) Overexpression of a peach defensin gene can enhance the activity of post-harvest biocontrol agents. Acta Hortic 682:1999–2006
Yeaman MR, Yount NY (2003) Mechanisms of antimicrobial peptide action and resistance. Pharmacol Rev 55:27–55. doi:10.1124/pr.55.1.2
Yevtushenko DP, Romero R, Forward BS, Hancock RE, Kay WW, Misra S (2005) Pathogen-induced expression of a cecropin A-melittin antimicrobial peptide gene confers antifungal resistance in transgenic tobacco. J Exp Bot 56:1685–1695. doi:10.1093/jxb/eri165
Yin ZX, He W, Chen WJ, Yan JH, Yang JN, Chan SM, He JG (2006) Cloning, expression and antimicrobial activity of an antimicrobial peptide, epinecidin-1, from the orange-spotted grouper, Epinephelus coioides. Aquaculture 253:204–211. doi:10.1016/j.aquaculture.2005.10.002
Yount NY, Yeaman MR (2005) Immunocontinuum: perspectives in antimicrobial peptide mechanisms of action and resistance. Protein Pept Lett 12:49–67. doi:10.2174/0929866053405959
Zakharchenko NS, Rukavtsova EB, Gudkov AT, Buryanov Ya I (2005) Enhanced resistance to phytopathogenic bacteria in transgenic tobacco plants with synthetic gene of antimicrobial peptide cecropin P1. Russ J Genet 41:1445–1452. doi:10.1007/s11177-005-0218-2
Zasloff M (2002) Antimicrobial peptides of multicellular organisms. Nature 415:389–395. doi:10.1038/415389a
Zhang G, Ross CR, Blecha F (2000) Porcine antimicrobial peptides: new prospects for ancient molecules of host defense. Vet Res 31:277–296. doi:10.1051/vetres:2000121
Zhang JX, Zhang SF, Wang TD, Guo XJ, Hu RL (2007) Mammary gland expression of antibacterial peptide genes to inhibit bacterial pathogens causing mastitis. J Dairy Sci 90:5218–5225. doi:10.3168/jds.2007-0301
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Keymanesh, K., Soltani, S. & Sardari, S. Application of antimicrobial peptides in agriculture and food industry. World J Microbiol Biotechnol 25, 933–944 (2009). https://doi.org/10.1007/s11274-009-9984-7
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DOI: https://doi.org/10.1007/s11274-009-9984-7