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A C2-domain phospholipid-binding protein MdCAIP1 positively regulates salt and osmotic stress tolerance in apple

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Abstract

High salt restricts the growth and development of plants. A diverse range of genes are involved in the response to salt stress. Here we report that MdCAIP1 (C2-domain ABA Insensitive Protein1), a single C2-domain containing protein, has a conserved structure consisting of an eight-stranded anti-parallel β-sandwich. MdCAIP1 was able to bind to phospholipids in a Ca2+-dependent manner and localized to the plasma membrane. qRT-PCR analysis showed that MdCAIP1 was induced by abiotic stresses including salt, osmotic, and drought stress, as well as the hormone JA. Transgenic apple calli and Arabidopsis over-expressing MdCAIP1 were more tolerant to salt and osmotic stress, and had higher proline content and lower MDA content under these stressful conditions. Furthermore, MdCAIP1 could homodimerize. These results indicate that MdCAIP1 is a Ca2+-dependent phospholipid-binding protein, and implicate it in tolerance to salt and osmotic stress.

Key message

MdCAIP1, a Ca2+-dependent phospholipid-binding protein, could homodimerize and positively regulated salt and osmotic tolerance in plants.

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Abbreviations

ABA:

Abscisic acid

MS:

Murashige and Skoog

MDA:

Malondialdehyde

qRT-PCR:

Quantitative reverse transcription-PCR

Y2H:

Yeast two-hybrid

JA:

Jasmonic acid

SA:

Salicylic acid

ACC:

1-Aminocyclopropanecarboxylic acid

VC:

Vector control

ET:

Ethylene

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Acknowledgements

This work was supported by grants from National Natural Science Foundation of China (31430074, U1706202), the Ministry of Agriculture of China (CARS-28), Shandong Province (SDAIT-06-03), and the Ministry of Education of China (IRT15R42).

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  1. Xiao-Juan Liu and Yuan-Hua Dong equally contributed to the work.

Authors and Affiliations

  1. State Key Laboratory of Crop Biology, MOA Key Laboratory of Horticultural Crop Biology and Germplasm Innovation, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An, 271018, Shandong, China

    Xiao-Juan Liu, Yuan-Hua Dong, Xin Liu, Chun-Xiang You & Yu-Jin Hao

  2. State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy Forestry, Beijing, 100091, China

    Xiao-Juan Liu

  3. National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, No. 35, Qinghua East Road, Beijing, 100083, China

    Xin Liu

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Contributions

Y.J.H. planned and designed the research. X.J.L., Y.H.D. and X.L. performed experiments and analyzed the data. X.J.L., C.X.Y. and Y.J.H. wrote the manuscript.

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Correspondence to Yu-Jin Hao.

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Communicated by Konstantin V. Kiselev.

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11240_2019_1600_MOESM1_ESM.doc

Supplementary material 1 (DOC 3519 kb) Figure. S1 qRT-PCR analysis of the expression level of MdCAIP1 in MdCAIP1 transgenic calli (A) and Arabidopsis (B). Figure. S2 The PAL (A), PPO (B) and PO (C) activtities in MdCAIP1 transgenic calli and wild tpye.

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Liu, XJ., Dong, YH., Liu, X. et al. A C2-domain phospholipid-binding protein MdCAIP1 positively regulates salt and osmotic stress tolerance in apple. Plant Cell Tiss Organ Cult 138, 29–39 (2019). https://doi.org/10.1007/s11240-019-01600-y

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